Departments of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
Departments of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
STAR Protoc. 2024 Mar 15;5(1):102842. doi: 10.1016/j.xpro.2024.102842. Epub 2024 Jan 18.
Mitochondrial dynamic process is important for cell viability, metabolic activity, and mitochondria health. Here, we present a protocol for measuring mitochondrial size through immunofluorescence staining, confocal imaging, and analysis in ImageJ. We describe the steps for tissue processing, antigen retrieval, mitochondrial staining using an integrating immunofluorescence assay, and computerized image analysis to measure each mitochondrial size in mouse and human liver tissues. This protocol reduces tissue sample volume and processing time for the preparation of primary cells. For complete details on the use and execution of this protocol, please refer to Pearah et al..
线粒体动态过程对于细胞活力、代谢活性和线粒体健康都很重要。在这里，我们提供了一种通过免疫荧光染色、共聚焦成像和 ImageJ 分析来测量线粒体大小的方案。我们描述了组织处理、抗原修复、使用整合免疫荧光测定法进行线粒体染色以及计算机化图像分析以测量小鼠和人肝组织中每个线粒体大小的步骤。该方案减少了用于制备原代细胞的组织样本量和处理时间。有关此方案的使用和执行的完整详细信息，请参阅 Pearah 等人的研究。
