Department of Botany, Dr. A.P.J. Abdul Kalam Government College, Newtown, West Bengal, India.
Plant Biology Laboratory, Organic and Medicinal Chemistry Division, CSIR-Indian Institute of Chemical Biology, Kolkata India.
Plant Signal Behav. 2022 Dec 31;17(1):2047352. doi: 10.1080/15592324.2022.2047352.
Glutathione (GSH) is well known to play a crucial role in imparting resistance against various pathogen invasions. Nevertheless, the role of GSH in regulating miRNA-mediated defense response is yet to be explored. To decipher the GSH-mediated regulation of miRNA expression during necrotrophic infection in , wild-type Col-0 and , the transgenic line exhibiting enhanced GSH content, were infected with necrotrophic pathogen plants exhibited enhanced resistance as compared to wild-type. MiRNA next-generation sequencing (NGS) was performed to compare the miRNA expression in Col-0 and 1 leaves. Under control condition, differentially expressed 96 known miRNAs and 17 novel miRNAs viz. ath-miR8167f, ath-miR1886.3, ath-miR3932b-5p, etc. were identified. However, under infected condition, 73 known and 43 novel differentially expressed miRNAs viz. ath-miR5652, ath-miR160b, ath-miR865-5p, etc. were identified. Functional annotation and enrichment analysis revealed that several miRNAs that target defense-related genes like leucine-rich repeat protein kinase, MYB transcription factors, TCP8, etc. were down regulated in the line, which, in turn, relieves the repression of their target gene expression, leading to resistance against infection. Together, the present investigation suggests that GSH plays a decisive role in modulating the miRNA-mediated regulation of defense-related genes during pathogen invasion.
谷胱甘肽 (GSH) 众所周知在赋予对各种病原体入侵的抗性方面起着至关重要的作用。然而，GSH 在调节 miRNA 介导的防御反应中的作用尚未被探索。为了解析 GSH 在 坏死型感染中调节 miRNA 表达的机制，我们比较了野生型 Col-0 和 ，后者是一种表现出增强 GSH 含量的转基因系，与野生型相比， 植物表现出增强的抗性。进行了 miRNA 下一代测序 (NGS) 以比较 Col-0 和 1 叶片中的 miRNA 表达。在对照条件下，鉴定了 96 个已知 miRNA 和 17 个新 miRNA，例如 ath-miR8167f、ath-miR1886.3、ath-miR3932b-5p 等。然而，在感染条件下，鉴定了 73 个已知和 43 个新的差异表达 miRNA，例如 ath-miR5652、ath-miR160b、ath-miR865-5p 等。功能注释和富集分析表明，一些靶向防御相关基因（如富含亮氨酸重复蛋白激酶、MYB 转录因子、TCP8 等）的 miRNA 在 系中下调，这反过来又解除了对其靶基因表达的抑制，导致对感染的抗性。总之，本研究表明 GSH 在调节病原体入侵过程中防御相关基因的 miRNA 介导的调控中起决定性作用。
