College of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang, China.
Research Center of Henan Provincial Agricultural Biomass Resource Engineering and Technology, Nanyang, China.
Pol J Microbiol. 2022 Sep 24;71(3):463-471. doi: 10.33073/pjm-2022-041. eCollection 2022 Sep 1.
Daptomycin is a cyclolipopeptide antibiotic produced by . It is widely used to treat drug-resistant bacterial infections; however, daptomycin yield in wild strains is very low. To improve the daptomycin production by the strain BNCC 342432, a modified method of ribosome engineering with superposition of streptomycin resistance was adopted in this study. The highest-yield mutant strain SR-2620 was obtained by increasing streptomycin resistance of BNCC 342432, and achieved daptomycin production of 38.5 mg/l in shake-flask fermentation, 1.79-fold higher than the parent strain and its heredity stability was stable. The morphological characteristics of the two strains were significantly different, and the 440 base G of the gene in the mutant strain was deleted, which resulted in a frameshift mutation. Our results demonstrate that gradually increasing strain resistance to streptomycin was an effective breeding method to improve daptomycin yield in .
达托霉素是一种由 产生的环脂肽抗生素。它被广泛用于治疗耐药菌感染；然而，野生菌株中的达托霉素产量非常低。为了提高 BNCC 342432 菌株的达托霉素产量，本研究采用核糖体工程与链霉素抗性叠加的改良方法。通过提高 BNCC 342432 的链霉素抗性，获得了最高产突变株 SR-2620，在摇瓶发酵中达托霉素产量达到 38.5mg/L，比亲本菌株提高了 1.79 倍，遗传稳定性稳定。两株菌的形态特征有明显差异，突变株中 基因的 440 个碱基 G 缺失，导致移码突变。我们的结果表明，逐渐提高菌株对链霉素的抗性是提高 的达托霉素产量的有效育种方法。
