Zhang Kun, Ma Jing, Gangurde Sunil S, Hou Lei, Xia Han, Li Nana, Pan Jiaowen, Tian Ruizheng, Huang Huailing, Wang Xingjun, Zhang Yindong, Zhao Chuanzhi
College of Tropical Crops, Hainan University, Haikou, China.
Institute of Crop Germplasm Resources (Institute of Biotechnology), Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, China.
Front Plant Sci. 2022 Sep 16;13:992124. doi: 10.3389/fpls.2022.992124. eCollection 2022.
Cultivated peanut ( L.) is an important source of edible oil and protein. Peanut testa (seed coat) provides protection for seeds and serves as a carrier for diversity metabolites necessary for human health. There is significant diversity available for testa color in peanut germplasms. However, the kinds and type of metabolites in peanut testa has not been comprehensively investigated. In this study, we performed metabolite profiling using UPLC-MS/MS for four peanut germplasm lines with different testa colors, including pink, purple, red, and white. A total of 85 metabolites were identified in four peanuts. Comparative metabolomics analysis identified 78 differentially accumulated metabolites (DAMs). Some metabolites showed significant correlation with other metabolites. For instance, proanthocyanidins were positively correlated with cyanidin 3-O-rutinoside and malvin, and negatively correlated with pelargonidin-3-glucoside. We observed that the total proanthocyanidins are most abundant in pink peanut variety WH10. The red testa accumulated more isoflavones, flavonols and anthocyanidins compared with that in pink testa. These results provided valuable information about differential accumulation of metabolites in testa with different color, which are helpful for further investigation of the molecular mechanism underlying biosynthesis and accumulation of these metabolites in peanut.
栽培花生（ ）是食用油和蛋白质的重要来源。花生种皮为种子提供保护，并作为人类健康所需多种代谢物的载体。花生种质中种皮颜色存在显著差异。然而，花生种皮中代谢物的种类和类型尚未得到全面研究。在本研究中，我们使用超高效液相色谱-串联质谱法（UPLC-MS/MS）对四个具有不同种皮颜色的花生种质系进行了代谢物谱分析，包括粉色、紫色、红色和白色。在四种花生中总共鉴定出85种代谢物。比较代谢组学分析鉴定出78种差异积累代谢物（DAMs）。一些代谢物与其他代谢物显示出显著相关性。例如，原花青素与矢车菊素3-O-芸香糖苷和锦葵色素呈正相关，与天竺葵素-3-葡萄糖苷呈负相关。我们观察到粉色花生品种WH10中原花青素总量最为丰富。与粉色种皮相比，红色种皮积累了更多的异黄酮、黄酮醇和花青素。这些结果为不同颜色种皮中代谢物的差异积累提供了有价值的信息，有助于进一步研究花生中这些代谢物生物合成和积累的分子机制。
