Lönnroth P, Jansson P A, Smith U
Am J Physiol. 1987 Aug;253(2 Pt 1):E228-31. doi: 10.1152/ajpendo.1987.253.2.E228.
To evaluate the usefulness of the tissue-microdialysis technique in humans, the glucose concentration in the intercellular water space was measured in the abdominal subcutaneous region in healthy subjects. A 30 X 0.3 mm dialysis fiber with a 3,000 MW cutoff was used. The dialysis catheter was calibrated in vivo by perfusing it with isotonic saline and four to five different glucose concentrations (0-5 mM). The perfusate was collected in 6-min fractions. Regression analysis of the results of the calibration yielded the perfusate glucose concentration, which was in equilibrium with the surrounding tissue. Validation experiments showed that this value could be precisely measured and represented the intercellular glucose concentration. The recovery of glucose in the dialysate (dialysate glucose concentration/medium) during the calibrations in vivo was only approximately one-half of that in vitro (recovery factors 0.28 vs. 0.44, respectively). Under steady-state conditions, the intercellular glucose concentration was similar to the glucose levels in the cubital vein. It is concluded that this microdialysis technique is a useful tool allowing measurements of metabolically active substances in the intercellular water space in vivo provided that the calibrations are properly performed.
为评估组织微透析技术在人体中的实用性，对健康受试者腹部皮下区域细胞间水空间中的葡萄糖浓度进行了测量。使用了截留分子量为3000道尔顿的30×0.3毫米透析纤维。通过用等渗盐水和四至五种不同葡萄糖浓度（0 - 5 mM）灌注透析导管，在体内对其进行校准。收集6分钟份的灌流液。对校准结果进行回归分析得出与周围组织处于平衡状态的灌流液葡萄糖浓度。验证实验表明该值能够被精确测量，并代表细胞间葡萄糖浓度。体内校准期间透析液中葡萄糖的回收率（透析液葡萄糖浓度/介质）仅约为体外回收率的一半（回收率分别为0.28和0.44）。在稳态条件下，细胞间葡萄糖浓度与肘静脉中的葡萄糖水平相似。得出的结论是，只要校准操作得当，这种微透析技术是一种有用的工具，可用于在体内测量细胞间水空间中的代谢活性物质。
