通过淋巴细胞转化试验-记忆淋巴细胞免疫刺激试验检测41-kDa细菌鞭毛蛋白

Detection of 41-kDa bacterial flagellin protein by the lymphocyte transformation test-memory lymphocyte immunostimulation assay.

作者信息

Puri Basant K, Monro Jean A

机构信息

University of Winchester & CAR Cambridge, UK.

Breakspear Medical Group Hemel Hempstead, Hertfordshire, UK.

出版信息

Am J Clin Exp Immunol. 2022 Aug 15;11(4):72-77. eCollection 2022.

PMID:36187894

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9520187/

Abstract

BACKGROUND/OBJECTIVES: Diagnosis of human infection by various species of the bacterial genus is mainly reliant on serological testing, polymerase chain reaction (PCR) or culture but such serological tests have been reported to have heterogeneous sensitivities, while PCR and culture have been reported as being of modest diagnostic value. It has been suggested that the adjunctive use of the lymphocyte transformation test-memory lymphocyte immunostimulation assay (LTT-MELISA) may be helpful in this regard; however, the clinical usefulness of this assay has been questioned. The immunodominant 41-kDa flagellin protein almost always gives rise to a marked human antibody response following infection. It was therefore decided to determine whether the LTT-MELISA detects the human antibody response to this antigen.

METHODS

Blood samples from consecutive patients with possible borreliosis attending a clinic were independently tested by both Western blots and LTT-MELISA.

RESULTS

After omitting cases with indeterminate Western blot results and equivocal LTT-MELISA results, multiple linear regression modelling demonstrated that the 41-kDa flagellin immunoglobulin (Ig) M level was predictable from two LTT-MELISA variables ( = 5.981, = 0.005). Similarly, the corresponding 41-kDa IgG model also contained two LTT-MELISA variables ( = 3.700, = 0.031).

CONCLUSION

It is concluded that the LTT-MELISA appears to be able to detect the response to this antigen.

摘要

背景/目的：诊断人类感染细菌属的各种菌种主要依赖血清学检测、聚合酶链反应（PCR）或培养，但据报道此类血清学检测具有不同的敏感性，而PCR和培养的诊断价值有限。有人认为，辅助使用淋巴细胞转化试验-记忆淋巴细胞免疫刺激测定法（LTT-MELISA）在这方面可能会有所帮助；然而，该测定法的临床实用性受到质疑。免疫显性的41 kDa鞭毛蛋白在感染后几乎总会引发明显的人体抗体反应。因此，决定确定LTT-MELISA是否能检测人体对该抗原的抗体反应。

方法

对一家诊所连续就诊的可能患有莱姆病的患者的血样分别进行蛋白质印迹法和LTT-MELISA检测。

结果

在排除蛋白质印迹结果不确定和LTT-MELISA结果不明确的病例后，多元线性回归模型显示，41 kDa鞭毛蛋白免疫球蛋白（Ig）M水平可由两个LTT-MELISA变量预测（ = 5.981， = 0.005）。同样，相应的41 kDa IgG模型也包含两个LTT-MELISA变量（ = 3.700， = 0.031）。