Fang Yuanpeng, Du Qiaoli, Yang Qian, Jiang Junmei, Hou Xiaolong, Yang Zaifu, Zhao Degang, Li Xiangyang, Xie Xin
Key Laboratory of Agricultural Microbiology, College of Agriculture, Guizhou University, Guiyang, China.
State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, China.
Front Microbiol. 2022 Sep 15;13:942302. doi: 10.3389/fmicb.2022.942302. eCollection 2022.
The family is one of the main E3 ubiquitin ligase families in plants. The family has been characterized in several species. However, genome-wide gene identification and expression profiling of the family in response to abiotic stress in remain unclear. In this study, we broadly identified 68 genes in the sorghum genome, including 2 genes, and 1 typical (Ub fusion degradation 2) gene. The gene family was divided into eight subclasses based on homology and conserved domain characteristics. Evolutionary analysis identified 14, 66, and 82 collinear gene pairs in sorghum compared with arabidopsis, rice, and maize, respectively, and a unique tandem repeat pair (/) is present in the sorghum genome. Gene Ontology (GO) enrichment analysis showed that U-box proteins were mainly related to ubiquitination and modification, and various stress responses. Comprehensive analysis of promoters, expression profiling, and gene co-regulation networks also revealed that many sorghum genes may be correlated with multiple stress responses. In summary, our results showed that sorghum contains 68 genes, which may be involved in multiple abiotic stress responses. The findings will support future gene functional studies related to ubiquitination in sorghum.
该家族是植物中主要的E3泛素连接酶家族之一。该家族已在多个物种中得到表征。然而,关于该家族在应对非生物胁迫时的全基因组基因鉴定和表达谱分析在[具体物种]中仍不清楚。在本研究中,我们在高粱基因组中广泛鉴定出68个[家族名称]基因,包括2个[具体基因类型1]基因和1个典型的[具体基因类型2](泛素融合降解2)基因。基于同源性和保守结构域特征,该[家族名称]基因家族被分为八个亚类。进化分析分别鉴定出高粱与拟南芥、水稻和玉米相比有14、66和82个共线基因对,并且高粱基因组中存在一个独特的串联重复对(/)。基因本体论(GO)富集分析表明,U-box蛋白主要与泛素化和修饰以及各种应激反应相关。对启动子、表达谱和基因共调控网络的综合分析还表明,许多高粱[家族名称]基因可能与多种应激反应相关。总之,我们的结果表明高粱含有68个[家族名称]基因,它们可能参与多种非生物胁迫反应。这些发现将为未来高粱中与泛素化相关的基因功能研究提供支持。