Yao Xin, Lai Dili, Zhou Meiliang, Ruan Jingjun, Ma Chao, Wu Weijiao, Weng Wenfeng, Fan Yu, Cheng Jianping
College of Agronomy, Guizhou University, Guiyang, China.
Institute of Crop Science, Chinese Academy of Agriculture Science, Beijing, China.
Front Plant Sci. 2023 Aug 4;14:1163357. doi: 10.3389/fpls.2023.1163357. eCollection 2023.
The GATA family of transcription factors is zinc finger DNA binding proteins involved in a variety of biological processes, including plant growth and development and response to biotic/abiotic stresses, and thus play an essential role in plant response to environmental changes. However, the gene family of () has not been systematically analyzed and reported yet. Herein, we used a variety of bioinformatics methods and quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) to explore the evolution and function of the 33 genes identified. These genes, distributed on 10 chromosomes, are classified into four subfamilies (I-IV) containing one pair of tandem duplications and nine pairs of segment duplications, which are more closely related to the monocot and genes. The physicochemical properties of the SbGATAs are significantly different among the subfamilies, while the protein structure and conserved protein motifs are highly conserved in the subfamilies. In addition, the transcription of SbGATAs is tissue-specific during growth and development, which allows for functional diversity in response to stress and hormones. Collectively, our study lays a theoretical foundation for an in-depth analysis of the functions, mechanisms and evolutionary relationships of SbGATA during plant growth and development.
GATA转录因子家族是锌指DNA结合蛋白，参与多种生物学过程，包括植物生长发育以及对生物/非生物胁迫的响应，因此在植物对环境变化的响应中发挥着重要作用。然而，（）的基因家族尚未得到系统分析和报道。在此，我们使用多种生物信息学方法和定量实时聚合酶链反应（qRT-PCR）来探究所鉴定的33个基因的进化和功能。这些基因分布在10条染色体上，分为四个亚家族（I-IV），包含一对串联重复和九对片段重复，它们与单子叶植物的和基因关系更为密切。SbGATA的理化性质在亚家族间存在显著差异，而蛋白质结构和保守蛋白基序在亚家族中高度保守。此外，SbGATA的转录在生长发育过程中具有组织特异性，这使其在应对胁迫和激素时具有功能多样性。总的来说，我们的研究为深入分析SbGATA在植物生长发育过程中的功能、机制和进化关系奠定了理论基础。
