Institute for Animal Nutrition, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, D 30173 Hannover, Germany.
Institute for Food Quality and Food Safety, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, D 30173 Hannover, Germany.
Microb Genom. 2022 Oct;8(10). doi: 10.1099/mgen.0.000874.
Campylobacteriosis is still the most commonly reported zoonosis in the European Union causing gastrointestinal disease in humans. One of the most common sources for these food-borne infections is broiler meat. Interactions between (.) and the intestinal microbiota might influence colonization in chickens. The aim of the present study was to gain further knowledge about exclusive interactions of the host microbiota with in -specific phage-free chickens under standardized conditions and special biosafety precautions.Therefore, 12 artificially infected ( inoculum with a challenge dose of 7.64 log c.f.u.) and 12 control chickens of the breed Ross 308 were kept under special biosafety measures in an animal facility. At day 42 of life, microbiota studies were performed on samples of caecal digesta and mucus. No -specific phages were detected by real-time PCR analysis of caecal digesta of control or artificially infected chickens. Amplification of the 16S rRNA gene was performed within the hypervariable region V4 and subsequently sequenced with Illumina MiSeq platform. R (version 4.0.2) was used to compare the microbiota between -negative and -positive chickens. The factor chickens' infection status contributed significantly to the differences in microbial composition of mucosal samples, explaining 10.6 % of the microbiota variation (=0.007) and in digesta samples, explaining 9.69 % of the microbiota variation (=0.015). The strongest difference between -non-infected and -infected birds was observed for the family whose presence in -infected birds could not be demonstrated. Further, several genera of the family appeared to be depressed in its abundance due to infection. A negative correlation was found between R-7 group and in -colonised chickens, both genera potentially competing for substrate. This makes R-7 group highly interesting for further studies that aim to find control options for infections and assess the relevance of this finding for chicken health and colonization.
空肠弯曲菌病仍然是欧盟最常见的报告动物源传染病，可引起人类胃肠道疾病。这些食源性病原体的最常见来源之一是肉鸡。（.)与肠道微生物群之间的相互作用可能会影响鸡的定植。本研究的目的是在标准化条件和特殊生物安全措施下，进一步了解宿主微生物群与特定噬菌体阴性鸡之间的排他性相互作用。因此，在特殊生物安全措施下，在动物设施中饲养了 12 只人工感染（接种 7.64 log c.f.u. 的挑战剂量）和 12 只罗斯 308 鸡对照鸡。在生命的第 42 天，对盲肠内容物和粘液样本进行了微生物组研究。通过实时 PCR 分析对照或人工感染鸡的盲肠内容物，未检测到 -特异性噬菌体。扩增 16S rRNA 基因在高度可变区 V4 内进行，随后使用 Illumina MiSeq 平台进行测序。R（版本 4.0.2）用于比较 -阴性和 -阳性鸡之间的微生物群。鸡感染状态这一因素对粘膜样本微生物组成的差异有显著贡献，解释了 10.6%的微生物群变化（=0.007），对粪便样本的解释为 9.69%的微生物群变化（=0.015）。-非感染和 -感染鸟类之间最强的差异观察到家族的存在。进一步的，家族的几个属由于 感染而在丰度上减少。在 -定植的鸡中发现了 R-7 组和 之间的负相关，这两个属可能在争夺底物。这使得 R-7 组非常有趣，可用于进一步的研究，旨在寻找控制 感染的选项，并评估这一发现对鸡健康和定植的相关性。
