Phodé, Z.I Albipôle, Terssac, France.
Biochimie et Toxicologie des Substances Biologiques (BTSB), Université de Toulouse, INU Champollion, Albi, France.
J Appl Microbiol. 2022 Jun;132(6):4501-4516. doi: 10.1111/jam.15521. Epub 2022 Mar 21.
This study was conducted to test the ability of a carvacrol-based formulation (Phodé, France) to decrease the C. jejuni caecal load in inoculated broiler chickens and to study the impact of the C. jejuni inoculation alone or combined with the product, on the caecal microbiota.
On day 1, chickens were either fed a control feed or the same diet supplemented with a carvacrol-based product. On day 21, the carvacrol-supplemented chickens and half of the non-supplemented chickens were inoculated with C. jejuni (10 CFU). Quantitative PCR was used to quantify C. jejuni in chicken caecal samples and 16S rRNA gene sequencing was carried out at 25, 31 and 35 days of age. A significant decrease of 1.4 log of the C. jejuni caecal load was observed in 35-day-old chickens supplemented with the product, compared to the inoculated and unsupplemented group (p < 0.05). The inoculation with C. jejuni significantly increased the population richness, Shannon and Simpson diversity and altered beta-diversity. Compared to the control group, the C. jejuni inoculation causes significant changes in the microbiota. The carvacrol-based product associated with C. jejuni inoculation increased the diversity and strongly modified the structure of the microbial community. Functional analysis by 16S rRNA gene-based predictions further revealed that the product up-regulated the pathways involved in the antimicrobial synthesis, which could explain its shaping effect on the caecal microbiota.
Our study confirmed the impairment of the caecal bacterial community after inoculation and demonstrated the ability of the product to reduce the C. jejuni load in chickens. Further investigations are needed to better understand the mode of action of this product to promote the installation of a beneficial microbiota to its host.
Results suggested that this product could be promising to control C. jejuni contamination of broilers.
本研究旨在测试香芹酚制剂（法国 Phodé）降低接种鸡盲肠中空肠弯曲杆菌负荷的能力，并研究空肠弯曲杆菌单独接种或与产品联合接种对盲肠微生物群的影响。
第 1 天，鸡要么喂食对照饲料，要么喂食添加香芹酚制剂的相同饲料。第 21 天，添加香芹酚的鸡和一半未添加的鸡接种空肠弯曲杆菌（10 CFU）。使用定量 PCR 定量鸡盲肠样品中的空肠弯曲杆菌，在 25、31 和 35 日龄进行 16S rRNA 基因测序。与接种且未添加的组相比，添加产品的 35 日龄鸡空肠负荷下降了 1.4 个对数（p<0.05）。空肠弯曲杆菌接种显著增加了种群丰富度、香农和辛普森多样性，并改变了β多样性。与对照组相比，空肠弯曲杆菌接种导致微生物群发生显著变化。香芹酚制剂与空肠弯曲杆菌接种联合使用增加了多样性，并强烈改变了微生物群落的结构。通过基于 16S rRNA 基因预测的功能分析进一步表明，该产品上调了参与抗菌合成的途径，这可以解释其对盲肠微生物群的塑造作用。
本研究证实了接种后盲肠细菌群落受损，并证明了该产品降低鸡中空肠弯曲杆菌负荷的能力。需要进一步研究以更好地了解该产品的作用机制，以促进有益微生物群定植到宿主。
结果表明，该产品有望控制肉鸡空肠弯曲杆菌污染。
