Department of Bioengineering, Stanford University, Stanford, CA, USA.
Department of Electrical Engineering, Stanford University, Stanford, CA, USA.
Nat Rev Neurosci. 2022 Nov;23(11):683-704. doi: 10.1038/s41583-022-00634-0. Epub 2022 Oct 3.
The past decade has witnessed remarkable advances in the simultaneous measurement of neuronal activity across many brain regions, enabling fundamentally new explorations of the brain-spanning cellular dynamics that underlie sensation, cognition and action. These recently developed multiregion recording techniques have provided many experimental opportunities, but thoughtful consideration of methodological trade-offs is necessary, especially regarding field of view, temporal acquisition rate and ability to guarantee cellular resolution. When applied in concert with modern optogenetic and computational tools, multiregion recording has already made possible fundamental biological discoveries - in part via the unprecedented ability to perform unbiased neural activity screens for principles of brain function, spanning dozens of brain areas and from local to global scales.
在过去的十年中，人们在同时测量多个脑区的神经元活动方面取得了显著进展，从而能够以前所未有的方式探索感觉、认知和行动所依赖的跨脑区细胞动力学。这些新开发的多区域记录技术提供了许多实验机会，但需要仔细考虑方法学上的权衡，尤其是在视野、时间采集率和保证细胞分辨率方面。当与现代光遗传学和计算工具结合使用时，多区域记录已经实现了一些基本的生物学发现——部分原因是具有前所未有的能力来进行无偏的神经活动筛选，以研究大脑功能的原理，这些研究涵盖了数十个脑区，并从局部扩展到全局尺度。
