Choi Seong Jin, Jung Jongtak, Kim Eu Suk, Kim Hong Bin, Park Jeong Su, Park Kyoung Un, Lee Hyunju, Lee Eunyoung, Choe Pyoeng Gyun, Kim Ji-Yeon, Lee Eun Joo, Song Kyoung-Ho
Department of Internal Medicine, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, Korea.
Department of Internal Medicine, Soonchunhyang University Seoul Hospital, Soonchunhyang University College of Medicine, Seoul, Korea.
Infect Chemother. 2022 Sep;54(3):517-528. doi: 10.3947/ic.2022.0081.
Self-sampling procedures to detect severe acute respiratory syndrome coronavirus 2 is important for patients who have difficulty visiting the hospital and may decrease the burden for health care workers (HCWs). The objective of this study was to evaluate the diagnostic performance, stability and usability of self-collected nasal and oral combo swabs and saliva specimens.
We conducted a case-control study with 50 patients with coronavirus disease 2019 (COVID-19) and 50 healthy volunteers from March, 2021 to June, 2021. We performed real-time reverse-transcription polymerase chain reaction to compare the diagnostic performance of self-collected specimens using positive percent agreements (PPAs).
The PPAs between self-collected and HCW-collected specimens were 77.3 - 81.0% and 80.5 -86.7% for the combo swabs and saliva specimens, respectively. The PPAs increased to 88.9 - 89.2% and 81.2 - 82.1% with a cycle threshold value ≤30.
The diagnostic performance of self sampling was comparable to that of HCW sampling in patients with high viral loads and may thus assist in the early diagnosis of COVID-19.
对于就医困难的患者而言，检测严重急性呼吸综合征冠状病毒2的自我采样程序很重要，并且可能减轻医护人员的负担。本研究的目的是评估自我采集的鼻拭子和口拭子组合样本以及唾液样本的诊断性能、稳定性和可用性。
我们在2021年3月至2021年6月期间对50例2019冠状病毒病（COVID-19）患者和50名健康志愿者进行了一项病例对照研究。我们进行了实时逆转录聚合酶链反应，以使用阳性百分比一致性（PPA）比较自我采集样本的诊断性能。
对于鼻拭子和口拭子组合样本以及唾液样本，自我采集样本与医护人员采集样本之间的PPA分别为77.3 - 81.0%和80.5 - 86.7%。当循环阈值≤30时，PPA分别提高到88.9 - 89.2%和81.2 - 82.1%。
在病毒载量高的患者中，自我采样的诊断性能与医护人员采样相当，因此可能有助于COVID-19的早期诊断。
