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GATA 因子 ELT-3 在线虫 An. gharbia 中指定内胚层，在一个祖先基因网络中。

The GATA factor ELT-3 specifies endoderm in Caenorhabditis angaria in an ancestral gene network.

机构信息

Department of Molecular, Cell and Systems Biology, University of California, Riverside, CA 92521, USA.

Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Miyazaki 889-1692, Japan.

出版信息

Development. 2022 Nov 1;149(21). doi: 10.1242/dev.200984. Epub 2022 Oct 24.

DOI:10.1242/dev.200984
PMID:36196618
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9720673/
Abstract

Endoderm specification in Caenorhabditis elegans occurs through a network in which maternally provided SKN-1/Nrf, with additional input from POP-1/TCF, activates the GATA factor cascade MED-1,2→END-1,3→ELT-2,7. Orthologues of the MED, END and ELT-7 factors are found only among nematodes closely related to C. elegans, raising the question of how gut is specified in their absence in more distant species in the genus. We find that the C. angaria, C. portoensis and C. monodelphis orthologues of the GATA factor gene elt-3 are expressed in the early E lineage, just before their elt-2 orthologues. In C. angaria, Can-pop-1(RNAi), Can-elt-3(RNAi) and a Can-elt-3 null mutation result in a penetrant 'gutless' phenotype. Can-pop-1 is necessary for Can-elt-3 activation, showing that it acts upstream. Forced early E lineage expression of Can-elt-3 in C. elegans can direct the expression of a Can-elt-2 transgene and rescue an elt-7 end-1 end-3; elt-2 quadruple mutant strain to viability. Our results demonstrate an ancestral mechanism for gut specification and differentiation in Caenorhabditis involving a simpler POP-1→ELT-3→ELT-2 gene network.

摘要

秀丽隐杆线虫的内胚层特化是通过一个网络实现的，在这个网络中，母源提供的 SKN-1/Nrf 与额外的 POP-1/TCF 输入一起激活 GATA 因子级联 MED-1、2→END-1、3→ELT-2、7。MED、END 和 ELT-7 因子的同源物仅存在于与秀丽隐杆线虫密切相关的线虫中，这就提出了一个问题，即在这些更远缘的属种中，肠道是如何在它们缺乏的情况下特化的。我们发现，C. angaria、C. portoensis 和 C. monodelphis 中的 GATA 因子基因 elt-3 的同源物在早期 E 谱系中表达，就在它们的 elt-2 同源物之前。在 C. angaria 中，Can-pop-1(RNAi)、Can-elt-3(RNAi)和一个 Can-elt-3 缺失突变导致一个明显的“无肠”表型。Can-pop-1 是 Can-elt-3 激活所必需的，表明它在其上游起作用。在秀丽隐杆线虫中强制早期 E 谱系表达 Can-elt-3 可以指导 Can-elt-2 转基因的表达，并拯救 elt-7 end-1 end-3;elt-2 四重突变体菌株的活力。我们的结果表明，涉及更简单的 POP-1→ELT-3→ELT-2 基因网络的 Caenorhabditis 肠道特化和分化的一个古老机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11fd/9720673/b1a4f49c964f/develop-149-200984-g9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11fd/9720673/0faf4824087c/develop-149-200984-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11fd/9720673/260940eac3e9/develop-149-200984-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11fd/9720673/f7737142deb3/develop-149-200984-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11fd/9720673/5b9b124d95d1/develop-149-200984-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11fd/9720673/62d9cc6f2942/develop-149-200984-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11fd/9720673/1d0acaf4af90/develop-149-200984-g6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11fd/9720673/7beaf1678a2d/develop-149-200984-g7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11fd/9720673/e15f09a3cdc8/develop-149-200984-g8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11fd/9720673/b1a4f49c964f/develop-149-200984-g9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11fd/9720673/0faf4824087c/develop-149-200984-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11fd/9720673/260940eac3e9/develop-149-200984-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11fd/9720673/f7737142deb3/develop-149-200984-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11fd/9720673/5b9b124d95d1/develop-149-200984-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11fd/9720673/62d9cc6f2942/develop-149-200984-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11fd/9720673/1d0acaf4af90/develop-149-200984-g6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11fd/9720673/7beaf1678a2d/develop-149-200984-g7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11fd/9720673/e15f09a3cdc8/develop-149-200984-g8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11fd/9720673/b1a4f49c964f/develop-149-200984-g9.jpg

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BMC Genomics. 2021 Dec 11;22(1):889. doi: 10.1186/s12864-021-08189-7.
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