Bukoski R D, Xue H, McCarron D A
Biochem Biophys Res Commun. 1987 Aug 14;146(3):1330-5. doi: 10.1016/0006-291x(87)90795-9.
The effect of several regulators of whole animal Ca2+ homeostasis on 45Ca uptake by primary cultures of aortic myocytes isolated from spontaneously hypertensive (SHR) and normotensive Wistar Kyoto (WKY) rats was examined. Exposure of confluent cells to 1.0, 1.25 or 1.50 mM ionized Ca2+ in serum-free medium for seven days resulted in increased 45Ca uptake at the higher concentrations of Ca2+ in cells of the SHR but not the WKY. 1,25 (OH)2 vitamin D3 (1 ng/ml) for 7 days caused enhanced influx in cells from both the SHR and WKY while parathyroid hormone (1-34) (1 ng/ml) was without effect. The data indicate that humoral factors that serve to regulate whole animal Ca2+ homeostasis may also play a role in the regulation of Ca2+ metabolism of the vascular smooth muscle cell.
研究了几种调节动物整体钙稳态的调节剂对从自发性高血压（SHR）大鼠和血压正常的Wistar Kyoto（WKY）大鼠分离的主动脉肌细胞原代培养物摄取45Ca的影响。将汇合细胞在无血清培养基中暴露于1.0、1.25或1.50 mM离子钙7天，结果显示，在较高钙浓度下，SHR细胞的45Ca摄取增加，而WKY细胞则未增加。1,25（OH）2维生素D3（1 ng/ml）处理7天可增强SHR和WKY细胞的钙内流，而甲状旁腺激素（1-34）（1 ng/ml）则无此作用。数据表明，调节动物整体钙稳态的体液因子可能在血管平滑肌细胞钙代谢的调节中也发挥作用。
