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基于RNA测序的慢传输型便秘关键基因分析

Analysis of Key Genes for Slow Transit Constipation Based on RNA Sequencing.

作者信息

Yu Linfeng, Yang Xiuding, Guan Wenlong, Zhang Dongxu, Ren Shuo, Xing Yanwei, An Da, Zhang Jian, Zhu Yuekun, Zhu Anlong

机构信息

Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China.

Department of Gastrointestinal Surgery, Sichuan Cancer Hospital, Chengdu, People's Republic of China.

出版信息

Int J Gen Med. 2022 Sep 28;15:7569-7579. doi: 10.2147/IJGM.S380208. eCollection 2022.

DOI:10.2147/IJGM.S380208
PMID:36199586
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9528044/
Abstract

PURPOSE

This study aims to identify key genes in slow transit constipation (STC). We also sought to explore the potential link between STC and colorectal cancer.

PATIENTS AND METHODS

mRNA expression profiles were obtained by RNA sequencing, and differentially expressed genes were identified. Functional enrichment analysis and a protein-protein interaction (PPI) network was explored, and differentially expressed genes common to STC and colorectal cancer were examined. Analysis of the effect of constipation and colorectal cancer common genes on the overall survival of colorectal cancer patients based on GEPIA database.

RESULTS

Functional enrichment showed that significantly different genes are related to lymphocyte chemotaxis, positive regulation of inflammatory response, cellular response to tumor necrosis factor, extracellular region, extracellular space and chemokine activity. The hub gene for STC was found in the PPI network. In addition, AQP8 and CFD were common differential genes for STC and colorectal cancer. AQP8 affects overall survival in patients with colorectal cancer.

CONCLUSION

Our findings will contribute to understanding the pathology of STC at the molecular level, with the first discovery that AQP8 may be a hub gene in the transition from STC to colorectal cancer.

摘要

目的

本研究旨在确定慢传输型便秘（STC）中的关键基因。我们还试图探索STC与结直肠癌之间的潜在联系。

患者与方法

通过RNA测序获得mRNA表达谱，并鉴定差异表达基因。进行功能富集分析并构建蛋白质-蛋白质相互作用（PPI）网络，检查STC和结直肠癌共有的差异表达基因。基于GEPIA数据库分析便秘和结直肠癌共同基因对结直肠癌患者总生存期的影响。

结果

功能富集表明，显著差异基因与淋巴细胞趋化性、炎症反应的正调控、细胞对肿瘤坏死因子的反应、细胞外区域、细胞外空间和趋化因子活性有关。在PPI网络中发现了STC的枢纽基因。此外，水通道蛋白8（AQP8）和补体因子D（CFD）是STC和结直肠癌共有的差异基因。AQP8影响结直肠癌患者的总生存期。

结论

我们的研究结果将有助于在分子水平上理解STC的病理，首次发现AQP8可能是从STC转变为结直肠癌过程中的枢纽基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cf4/9528044/2172a638096b/IJGM-15-7569-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cf4/9528044/4475a4a88118/IJGM-15-7569-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cf4/9528044/5df21b58f990/IJGM-15-7569-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cf4/9528044/252fc4c86cd7/IJGM-15-7569-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cf4/9528044/bbb27a3c0e87/IJGM-15-7569-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cf4/9528044/e53a938e762f/IJGM-15-7569-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cf4/9528044/2172a638096b/IJGM-15-7569-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cf4/9528044/4475a4a88118/IJGM-15-7569-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cf4/9528044/5df21b58f990/IJGM-15-7569-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cf4/9528044/252fc4c86cd7/IJGM-15-7569-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cf4/9528044/bbb27a3c0e87/IJGM-15-7569-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cf4/9528044/e53a938e762f/IJGM-15-7569-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cf4/9528044/2172a638096b/IJGM-15-7569-g0006.jpg

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The Activation of Prothrombin Seems to Play an Earlier Role than the Complement System in the Progression of Colorectal Cancer: A Mass Spectrometry Evaluation.凝血酶原的激活在结直肠癌进展中似乎比补体系统发挥更早的作用：一项质谱评估。
Diagnostics (Basel). 2020 Dec 11;10(12):1077. doi: 10.3390/diagnostics10121077.
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Long-Term Risks of Parkinson's Disease, Surgery, and Colorectal Cancer in Patients With Slow-Transit Constipation.
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Clin Gastroenterol Hepatol. 2021 Dec;19(12):2577-2586.e6. doi: 10.1016/j.cgh.2020.08.059. Epub 2020 Aug 31.
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Maren Pills Improve Constipation via Regulating AQP3 and NF-B Signaling Pathway in Slow Transit Constipation In Vitro and In Vivo.马伦丸通过在体外和体内调节慢传输型便秘中的水通道蛋白3（AQP3）和核因子-κB（NF-κB）信号通路改善便秘。
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