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使用低共熔溶剂水溶液通过高压脉冲辅助从木棉中提取黄酮类化合物

High-voltage pulse-assisted extraction of flavonoids from kapok using deep eutectic solvent aqueous solutions.

作者信息

Wei Ruijing, Hu Lu, Wang Lihua, Yan Peng, Lin Tao, Wang Ning, Sun Huaiqing, Zheng Bisheng, Guo Chaowan

机构信息

Research and Development Center, Guangdong Marubi Biotechnology Co., Ltd. 510530 Guangdong China

School of Food Science and Engineering, South China University of Technology 381 Wushan Road Guangzhou 510640 Guangdong China.

出版信息

RSC Adv. 2022 Sep 2;12(38):25025-25034. doi: 10.1039/d2ra03969a. eCollection 2022 Aug 30.

DOI:10.1039/d2ra03969a
PMID:36199877
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9437896/
Abstract

In this study, deep eutectic solvents coupled with a pulsed electric field (PEF-DES) were first applied to the extraction of traditional Chinese medicine plants. This study uses the PEF-DES extraction technique to extract TG-KF (Kapok flavonoid solution extracted with DES-TG). PEF-DES is a simple, effective and environmentally-friendly technology and can be used in industrial-scale production. For the optimal extraction conditions of TG-KF, DES-TG was used as a solvent, the DES-TG concentration was 50%, the solid-liquid ratio was 1 : 30, the electric field intensity was 0.55 kV cm, the number of pulses was 100, and the yield of flavonoids was 14.36 ± 0.35%. TG-KF has very good stability and there is no precipitation or discoloration within 6 months. The results of chicken embryo experiments and human patch tests show that 10% TG-KF aqueous solution has no irritation. DPPH experiments show that TG-KF has excellent efficacy as an antioxidant. Overall, TG-KF is expected to become a potential antioxidant raw material.

摘要

在本研究中，首次将深共熔溶剂与脉冲电场（PEF-DES）相结合应用于中药植物的提取。本研究采用PEF-DES提取技术提取TG-KF（用DES-TG提取的木棉黄酮溶液）。PEF-DES是一种简单、有效且环保的技术，可用于工业规模生产。对于TG-KF的最佳提取条件，以DES-TG为溶剂，DES-TG浓度为50%，固液比为1∶30，电场强度为0.55 kV/cm，脉冲数为100，黄酮类化合物的产率为14.36±0.35%。TG-KF具有非常好的稳定性，6个月内无沉淀或变色现象。鸡胚实验和人体斑贴试验结果表明，10%的TG-KF水溶液无刺激性。DPPH实验表明，TG-KF作为抗氧化剂具有优异的功效。总体而言，TG-KF有望成为一种潜在的抗氧化剂原料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a17/9437896/b42f4e98d06d/d2ra03969a-f7.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a17/9437896/c56080a4bd08/d2ra03969a-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a17/9437896/7f83be136d27/d2ra03969a-f2.jpg
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