He Xin, Ma RuLin, Li Yu, Wang Haixia, Yan YiZhong, Mao YiDan, Liao ShengYu, Sun XueYing, Guo ShuXia, Guo Heng
Department of Public Health, Shihezi University School of Medicine, North 2th Road, Shihezi 832000, Xinjiang, China.
NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China.
Int J Hypertens. 2022 Sep 26;2022:4632087. doi: 10.1155/2022/4632087. eCollection 2022.
This study aims to analyze the expressions of miR-21, miR-29, and miR-199 in the serum of the patients with H-type hypertension among Kazakhs. Then, we analyzed the effect of MTHFR 677C > T polymorphism on the association between the above miRNA and H-type hypertension.
In this study, the expression of miR-21, miR-29, and miR-199 was quantitatively measured in 120 serum samples and then stratified according to the C677T polymorphism to analyze the relationship between target miRNAs and HHcy.
The expression of miR-21/-29 in the hypertension group was higher than the normal group ( < 0.001). And the expression of miR-199 was higher in the hcy group than in the normal group ( < 0.001). In the CC and CT genotypes of MTHFR 677C > T, the expression of miR-21 was lower in the HHcy patients than in the normal individuals ( = 0.005 and = 0.001) and miR-199 was significantly higher in the HHcy patients than in the normal ones ( = 0.002 and = 0.048). No such difference was found in the TT genotype. Logistic regression analysis showed that after adjusting for sex, age, BMI, systolic blood pressure, diastolic blood pressure, and MTHFRC677 T gene polymorphism, miR-21 was negatively correlated with hcy (OR = 0.222, 95% CI (0.101-0.485), < 0.001) and miR-199 was positively correlated with hcy (OR = 1.823,95%CI (1.272∼2.614), = 0.001).
miR-21, miR-29, and miR-199 are associated with H-type hypertension in the Kazakhs, especially hyperhomocysteinemia. And these three miRNAs may serve as biomarkers to provide clues to the potential pathogenesis of H-type hypertension.
本研究旨在分析哈萨克族H型高血压患者血清中miR - 21、miR - 29和miR - 199的表达情况。然后，分析亚甲基四氢叶酸还原酶（MTHFR）677C＞T基因多态性对上述微小RNA（miRNA）与H型高血压之间关联的影响。
本研究对120份血清样本中miR - 21、miR - 29和miR - 199的表达进行定量检测，然后根据C677T基因多态性进行分层，以分析目标miRNA与高同型半胱氨酸血症（HHcy）之间的关系。
高血压组中miR - 21/-29的表达高于正常组（P＜0.001）。且同型半胱氨酸血症（hcy）组中miR - 199的表达高于正常组（P＜0.001）。在MTHFR 677C＞T的CC和CT基因型中，HHcy患者中miR - 21的表达低于正常个体（P = 0.005和P = 0.001），而HHcy患者中miR - 199的表达显著高于正常个体（P = 0.002和P = 0.048）。在TT基因型中未发现此类差异。Logistic回归分析显示，在调整性别、年龄、体重指数（BMI）、收缩压、舒张压和MTHFR C677T基因多态性后，miR - 21与hcy呈负相关（比值比（OR）= 0.222，95%置信区间（CI）（0.101 - 0.485），P＜0.001），miR - 199与hcy呈正相关（OR = 1.823，95%CI（1.272～2.614），P = 0.001）。
miR - 21、miR - 29和miR - 199与哈萨克族的H型高血压有关，尤其是与高同型半胱氨酸血症有关。这三种miRNA可能作为生物标志物，为H型高血压的潜在发病机制提供线索。
