Negroni Elisa, Kondili Maria, Muraine Laura, Bensalah Mona, Butler-Browne Gillian Sandra, Mouly Vincent, Bigot Anne, Trollet Capucine
Sorbonne Université, Inserm, Institut de Myologie, Centre de Recherche en Myologie, Paris, France.
Front Cell Dev Biol. 2022 Sep 19;10:952041. doi: 10.3389/fcell.2022.952041. eCollection 2022.
Skeletal muscle is a highly plastic tissue composed of a number of heterogeneous cell populations that, by interacting and communicating with each other, participate to the muscle homeostasis, and orchestrate regeneration and repair in healthy and diseased conditions. Although muscle regeneration relies on the activity of muscle stem cells (MuSCs), many other cellular players such as inflammatory, vascular and tissue-resident mesenchymal cells participate and communicate with MuSCs to sustain the regenerative process. Among them, Fibro-Adipogenic Progenitors (FAPs), a muscle interstitial stromal population, are crucial actors during muscle homeostasis and regeneration, interacting with MuSCs and other cellular players and dynamically producing and remodelling the extra-cellular matrix. Recent emerging single-cell omics technologies have resulted in the dissection of the heterogeneity of each cell populations within skeletal muscle. In this perspective we have reviewed the recent single-cell omics studies with a specific focus on FAPs in mouse and human muscle. More precisely, using the OutCyte prediction tool, we analysed the "virtual" secretome of FAPs, in resting and regenerating conditions, to highlight the potential of RNAseq data for the study of cellular communication.
骨骼肌是一种高度可塑性的组织，由许多异质性细胞群体组成，这些细胞群体通过相互作用和交流，参与肌肉内环境稳态，并在健康和患病状态下协调再生和修复。尽管肌肉再生依赖于肌肉干细胞（MuSCs）的活性，但许多其他细胞成分，如炎性细胞、血管细胞和组织驻留间充质细胞，也参与其中并与MuSCs相互交流，以维持再生过程。其中，成纤维脂肪生成祖细胞（FAPs）是一种肌肉间质基质群体，在肌肉内环境稳态和再生过程中起着关键作用，它与MuSCs和其他细胞成分相互作用，并动态产生和重塑细胞外基质。最近出现的单细胞组学技术已导致对骨骼肌内每个细胞群体的异质性进行剖析。从这个角度出发，我们回顾了最近的单细胞组学研究，特别关注小鼠和人类肌肉中的FAPs。更确切地说，我们使用OutCyte预测工具，分析了FAPs在静息和再生条件下的“虚拟”分泌组，以突出RNAseq数据在细胞通讯研究中的潜力。
