Department of Engineering Science Graduate School of Informatics and Engineering, The University of Electro-Communications 1-5-1 Chofugaoka, Chofu, Tokyo, 182-8585, Japan.
Chembiochem. 2022 Nov 18;23(22):e202200360. doi: 10.1002/cbic.202200360. Epub 2022 Oct 26.
DNA programmable structures of various morphologies have attracted extensive attention due to their potential for materials science and biomedical applications. Here, we report the formation of micro-sized hexagons via assembly of only one pair of short double-stranded DNA in buffer-salt poly(ethylene glycol) solution. Each DNA strand had complementary bases with a two-base overhang. The procedure of heating and subsequent cooling of blunt-ended double-stranded DNA resulted in different assemblies. These results indicated that end-to-end adhesion at the terminals induced by complementary overhangs were required to construct the hexagonal DNA assemblies. The stable formation of the hexagons was highly dependent on heating temperature. In addition, concentration adjustments of DNA and poly(ethylene glycol) were essential. Circular dichroism spectral measurements and polarization microscopy observations indicated parallel alignment of double-stranded DNA in the hexagonal platelet. Self-assembled micro-sized hexagons composed of simple building blocks may have great potential for future biomedical device development.
由于其在材料科学和生物医学应用方面的潜力，各种形态的 DNA 可编程结构引起了广泛关注。在这里，我们报告了仅通过在缓冲盐聚乙二醇溶液中组装一对短双链 DNA 来形成微尺寸六边形。每条 DNA 链都具有互补碱基和两个碱基的突出端。加热和随后冷却钝端双链 DNA 的过程导致了不同的组装。这些结果表明，互补突出端在末端引起的端到端粘附对于构建六边形 DNA 组装体是必需的。六边形的稳定形成高度依赖于加热温度。此外，DNA 和聚乙二醇的浓度调整也是必不可少的。圆二色光谱测量和偏振显微镜观察表明，双链 DNA 在六边形薄片中呈平行排列。由简单结构单元组成的自组装微尺寸六边形可能在未来的生物医学设备开发中具有巨大的潜力。
