Chair of Chemistry of Biogenic Resources, Technical University of Munich, Campus Straubing for Biotechnology and Sustainability, Schulgasse 16, 94315 Straubing, Germany.
Catalysis Research Center, Technical University of Munich, 85748 Garching, Germany.
Chem Commun (Camb). 2022 Oct 25;58(85):11945-11948. doi: 10.1039/d2cc03423a.
Developing inexpensive nicotinamide cofactor biomimetics to replace the expensive NAD(P)/H cofactors is an ongoing research activity. Here we present mutational studies on a thermostable glucose dehydrogenase from (GDH) using a novel set of synthetic cofactors. Furthermore, we show the successful oxidation of a variety of different sugars in the context of cofactor regeneration. This combined approach resulted in an 160-fold improved system compared to the native enzyme with the standard biomimetic BNA. These findings pave the way towards competitive industrial utilization of artificial cofactor regeneration systems.
开发廉价的烟酰胺辅酶类似物来替代昂贵的 NAD(P)/H 辅酶是一项持续的研究活动。在这里，我们使用一组新的合成辅酶对来自Thermobifida fusca 的耐热葡萄糖脱氢酶 (GDH)进行了突变研究。此外，我们还展示了在辅酶再生的情况下，各种不同糖的成功氧化。与使用标准仿生 BNA 的天然酶相比，这种组合方法使系统的性能提高了 160 倍。这些发现为人工辅酶再生系统在工业上的竞争应用铺平了道路。
