Ramtin Maryam, Sharifniya Fariba, Larypoor Mohaddeseh, Mirpour Mirsasan, Zarrabi Saeid
Department of Microbiology, Faculty of Biological Sciences, Tehran North Branch, Islamic Azad University, Tehran, Iran.
Department of Biology, Faculty of Biological Sciences, Tehran North Branch, Islamic Azad University, Tehran, Iran.
Curr Microbiol. 2022 Oct 6;79(11):338. doi: 10.1007/s00284-022-03042-w.
Owing to the resistance of nosocomial pathogens to antibiotics, the need for herbal medicines is felt. The aim of this study was to identify the chemical composition of bark essential oils of Campsis radicans and the effect of its free and encapsulated form on resistant nosocomial pathogens. This plant is a native of Northern Iran. The Bark essential oils of Campsis radicans was first extracted and its antimicrobial effects were investigated. Then, its phytochemical compounds were determined using Gas Chromatography-Mass Spectrometry (GC/MS). Guaiacol (2-methoxy phenol) was selected as the active ingredient among 32 compounds (2.40%). It was encapsulated and the encapsulation efficiency (EE), the particle size, polydispersity index (pdi), Fourier transform infrared (FTIR), release, and stability were determined. Then, the antimicrobial effect of both free and encapsulated forms was evaluated on cotrimoxazole-resistant Pseudomonas aeruginosa, cefixime-resistant Escherichia coli, and fluconazole-resistant Candida albicans. It was observed that both free and encapsulated forms of Guaiacol had an antimicrobial effect on the studied resistant strains, but the encapsulated form had a more antimicrobial effect due to more stability and a more targeted effect. MBC (MFC) ranged from 0.270 to 0.439 µg/ml in the free form and from 0.055 to 0.133 µg/ml in the encapsulated form, EE was 86%, particle size, and pdi were 138 nm and 0.26, respectively. This study showed that this plant can be a suitable alternative to chemical drugs due to its antimicrobial effects.
由于医院病原体对抗生素产生耐药性，人们开始需要草药。本研究的目的是确定凌霄花树皮精油的化学成分及其游离形式和包封形式对耐药医院病原体的影响。这种植物原产于伊朗北部。首先提取了凌霄花的树皮精油，并研究了其抗菌效果。然后，使用气相色谱 - 质谱联用仪（GC/MS）测定其植物化学化合物。在32种化合物（2.40%）中选择愈创木酚（2 - 甲氧基苯酚）作为活性成分。对其进行包封，并测定包封效率（EE）、粒径、多分散指数（pdi）、傅里叶变换红外光谱（FTIR）、释放率和稳定性。然后，评估游离形式和包封形式对耐复方新诺明的铜绿假单胞菌、耐头孢克肟的大肠杆菌和耐氟康唑的白色念珠菌的抗菌效果。观察到愈创木酚的游离形式和包封形式对所研究的耐药菌株均有抗菌作用，但包封形式由于具有更高的稳定性和更具靶向性作用而具有更强的抗菌效果。游离形式的MBC（MFC）范围为0.270至0.439μg/ml，包封形式为0.055至0.133μg/ml，EE为86% ，粒径和pdi分别为138nm和0.26。本研究表明，由于其抗菌作用，这种植物可以成为化学药物的合适替代品。
