Cruz R M, Lambert P R, Rubel E W
Arch Otolaryngol Head Neck Surg. 1987 Oct;113(10):1058-62. doi: 10.1001/archotol.1987.01860100036017.
This study examines the temporal pattern of hair cell loss in the chick basilar papilla following ten days of gentamicin administration in hatchling chicks. Chicks were subsequently killed at ages 11, 18, 25, and 32 days. The basilar papillae were embedded in plastic and serially sectioned for light microscopic analysis. Hair cell counts were obtained at 100-microM intervals throughout the length of the papilla. Significant hair cell loss was documented basally in the 11-day-old chicks, and spread apically over time to maximal loss in the 18-day-old animals. Relative to the control chicks, there was a 36% hair cell loss in these animals. Interestingly, there appears to be a progressive partial recovery of the normal hair cell counts in the 25- and 32-day-old animals.
本研究检测了新生雏鸡连续10天给予庆大霉素后，其基底乳头中毛细胞损失的时间模式。随后在雏鸡11日龄、18日龄、25日龄和32日龄时将其处死。将基底乳头包埋于塑料中并进行连续切片，用于光学显微镜分析。在乳头全长每隔100微米获取毛细胞计数。在11日龄雏鸡的基底部位记录到显著的毛细胞损失，且随时间向顶部扩散，在18日龄动物中损失达到最大。相对于对照雏鸡，这些动物的毛细胞损失了36%。有趣的是，在25日龄和32日龄的动物中，正常毛细胞计数似乎有逐渐的部分恢复。
