School of Life Science, Liaoning Normal University, Dalian, 116029, China; Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China.
Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China.
Gene Expr Patterns. 2022 Dec;46:119280. doi: 10.1016/j.gep.2022.119280. Epub 2022 Oct 4.
Sea urchin (Mesocentrotus nudus) is an important economically mariculture species in several Asian countries, and gonads are the sole edible parts for people. In addition to commercial value, it is an excellent model for studying gonadal development, sex determination and sex differentiation. Identify sex-related genes is an effective way to reveal the molecular mechanism of gonadal development. In the present study, the foxl2 homologous gene was identified in M. nudus. Foxl2 is not a maternal factor, and is detected for the first time in two-arm stages. Additionally, the expression of foxl2 in the testis is higher than in the ovaries at the same developmental stages. The foxl2 transcripts were specifically enriched in the cytoplasm of germ cellsboth in the ovary and testis, but their proteins were more concentrated in the area near the oocyte nucleus. Overall, this study contributes to our understanding of the dynamic and sexually dimorphic expression pattern of foxl2 and provide a useful germ cell marker during gametogenesis in sea urchin.
海胆（光棘球海胆）是亚洲几个国家重要的经济养殖物种，其生殖腺是唯一可食用的部分。除了商业价值外，它还是研究性腺发育、性别决定和性别分化的理想模型。鉴定与性别相关的基因是揭示性腺发育分子机制的有效途径。本研究在光棘球海胆中鉴定到 foxl2 同源基因。Foxl2 不是母源性因子，并且首次在二腕幼虫期被检测到。此外，foxl2 在睾丸中的表达在相同发育阶段高于卵巢。foxl2 转录本在卵巢和睾丸的生殖细胞的细胞质中特异性富集，但它们的蛋白质在靠近卵母细胞核的区域更集中。总的来说，这项研究有助于我们理解 foxl2 的动态和性别二态性表达模式，并为海胆配子发生过程中的生殖细胞标记提供了有用的信息。
