Wang Quanchao, Liu Ying, Wang Yanxia, Jiang Shaoyu, Zhang Chuanxin, Li Baoquan
Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China.
Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, 266071, China.
Mar Biotechnol (NY). 2022 Mar;24(1):32-39. doi: 10.1007/s10126-021-10084-x. Epub 2021 Nov 23.
Mesocentrotus nudus is an economically important mariculture species. Identification of sex-related markers and candidate genes has potential implications for sex-control breeding of Mesocentrotus nudus. The aim of this study was to identify the molecular markers and genes associated with the sex of M. nudus. Initial GWAS analysis based on 80 individuals genotyped by using GBS identified 22 sex-related SNPs located on 9 GBS tags. Further targeted sequencing in another population of 124 individuals confirmed that 7 SNPs located on 7 GBS tags displayed complete associations with sex, suggesting a ZW/ZZ sex-determination system in M. nudus. Besides, genome and transcriptome annotations presented that the heat shock protein 75 kDa, mitochondrial (trap1), and protein furry homolog-like (fryl) may be important candidate genes involved in sex determination and differentiation in M. nudus. However, further studies are needed to elucidate the functions of these genes. Overall, the current results not only provide molecular markers that may contribute to the sex-control breeding for M. nudus, but also provide new insights to understand the regulatory mechanism of sea urchin sex.
光棘球海胆是一种具有重要经济价值的海水养殖物种。鉴定与性别相关的标记和候选基因对光棘球海胆的性别控制育种具有潜在意义。本研究的目的是鉴定与光棘球海胆性别相关的分子标记和基因。基于使用GBS对80个个体进行基因分型的初始全基因组关联研究（GWAS）分析，在9个GBS标签上鉴定出22个与性别相关的单核苷酸多态性（SNP）。在另一个由124个个体组成的群体中进行的进一步靶向测序证实，位于7个GBS标签上的7个SNP与性别完全相关，这表明光棘球海胆存在ZW/ZZ性别决定系统。此外，基因组和转录组注释显示，热休克蛋白75 kDa、线粒体（trap1）和类蛋白Furry同源物（fryl）可能是参与光棘球海胆性别决定和分化的重要候选基因。然而，需要进一步研究来阐明这些基因的功能。总体而言，目前的结果不仅提供了可能有助于光棘球海胆性别控制育种的分子标记，还为理解海胆性别的调控机制提供了新的见解。
