College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China.
Graduate Student Office, Nanjing Institute of Technology, Nanjing, 210037, Jiangsu, China.
Microb Pathog. 2022 Dec;173(Pt A):105813. doi: 10.1016/j.micpath.2022.105813. Epub 2022 Oct 3.
To complete the investigation of the bacterial inhibitory activity of ginkgolic acid (GA), the quorum sensing activity of GA was investigated to assess the potential of ginkgo biloba exocarp for the production of quorum sensing inhibitor. The effect of GA (C15:1) on the growth and secondary metabolism of p. aeruginosa PAO1 were tested. The results showed that GA (C15:1) had no effect on the growth of p. aeruginosa PAO1, and that GA (C15:1) was able to inhibit the formation of quorum sensing dependent virulence factors, but promoted the formation of biofilms. Finally, the above experimental results were verified by LasR-GFP, RhlR-GFP system and fluorescence quantitative PCR. These results suggested that GA (C15:1) was capable of modulating the quorum sensing system of p. aeruginosa PAO1 and possessed potential as an anti-virulence factor drug, but is not suitable for development as a broad-spectrum biofilm inhibitor.
为了完成对银杏酸（GA）抑菌活性的研究，考察了 GA 的群体感应活性，以评估银杏外种皮产生群体感应抑制剂的潜力。测试了 GA（C15：1）对铜绿假单胞菌 PAO1 生长和次级代谢的影响。结果表明，GA（C15：1）对铜绿假单胞菌 PAO1 的生长没有影响，GA（C15：1）能够抑制群体感应依赖性毒力因子的形成，但促进生物膜的形成。最后，通过 LasR-GFP、RhlR-GFP 系统和荧光定量 PCR 验证了上述实验结果。这些结果表明，GA（C15：1）能够调节铜绿假单胞菌 PAO1 的群体感应系统，具有作为抗毒力因子药物的潜力，但不适合开发为广谱生物膜抑制剂。
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