Hawkins R A, Sangster K, Krajewski A
Br J Cancer. 1987 Jun;55(6):611-6. doi: 10.1038/bjc.1987.124.
The presence or absence of an oestrogen receptor-related antigen in breast tumours has been examined histochemically using a monoclonal antibody ('Ds' - Coffer & King, 1981). In frozen sections, fixed either by the method of Tamura et al. (1980) or in methanol, staining was apparent in 14/24 (58%) and 22/26 (85%) of the breast cancers respectively. In paraffin sections fixed in ethanol, staining was present in 25/33 breast cancers (76%). In either type of section, staining was predominantly in the cytoplasm of the epithelial cells. When staining was scored by independent observers (2 or 3) and related to the tumour oestrogen receptor activity, determined by a standard biochemical technique, antigen was present in both receptor-positive and receptor-negative tumours. No significant association was found between the presence of antigen and receptors in the frozen sections, but for the series of paraffin sections, there was a weak association (r = +0.48) between the presence of the two proteins. Histochemical processing of paraffin sections from 9 tumours under conditions of higher sensitivity increased the staining significantly in 2/9 tumours, but did not alter the relationship between staining and receptor status. Six tissues were stained after exposure to 'receptor-translocating' conditions (25 degrees C/2 nM oestradiol/both for 1 h): this did not consistently change the subcellular staining pattern, though all tissues tended to stain more after exposure to 25 degrees C. Staining was not blocked by absorption of the D5 antiserum with a variety of pure proteins or human serum but at higher concentrations (approx. 2-15 mg protein ml-1), extracts from human uterus, an oestrogen-receptor-positive breast cancer and an oestrogen-receptor-negative breast cancer all effectively abolished staining in sections from another breast cancer. These results are consistent with other reports suggesting that the D5 antibody detects an antigen which is not the oestrogen receptor, but which may be associated with the receptor in its tissue distribution.
已使用单克隆抗体（“Ds”-Coffer和King，1981年）通过组织化学方法检测乳腺肿瘤中雌激素受体相关抗原的有无。在冷冻切片中，分别采用田村等人（1980年）的方法或甲醇固定，在14/24（58%）和22/26（85%）的乳腺癌中可见染色。在乙醇固定的石蜡切片中，25/33例乳腺癌（76%）存在染色。在任何一种切片类型中，染色主要位于上皮细胞的细胞质中。当由独立观察者（2名或3名）对染色进行评分并与通过标准生化技术测定的肿瘤雌激素受体活性相关联时，抗原在受体阳性和受体阴性肿瘤中均存在。在冷冻切片中未发现抗原与受体之间存在显著关联，但对于石蜡切片系列，两种蛋白质的存在之间存在弱关联（r = +0.48）。在更高灵敏度条件下对9个肿瘤的石蜡切片进行组织化学处理，2/9个肿瘤的染色显著增加，但未改变染色与受体状态之间的关系。6个组织在暴露于“受体转位”条件（25℃/2 nM雌二醇/两者均1小时）后进行染色：这并未一致地改变亚细胞染色模式，尽管所有组织在暴露于25℃后往往染色更多。用多种纯蛋白质或人血清吸收D5抗血清不能阻断染色，但在更高浓度（约2 - 15 mg蛋白质/ml-1）下，来自人子宫、雌激素受体阳性乳腺癌和雌激素受体阴性乳腺癌的提取物均能有效消除另一个乳腺癌切片中的染色。这些结果与其他报告一致，表明D5抗体检测到的抗原不是雌激素受体，但在组织分布上可能与受体相关。
