Girling A, Caleffi M, King R J, Millis R R
Clinical Oncology Unit, Imperial Cancer Research Fund, Guy's Hospital, London.
J Clin Pathol. 1988 Apr;41(4):448-53. doi: 10.1136/jcp.41.4.448.
Patterns of staining with a monoclonal antibody which recognises D5 antigen (a 29,000 kD oestrogen receptor-related protein) were studied in seven normal and 76 benign breast biopsy specimens as well as in 12 cases of pure in situ mammary carcinoma. Staining in benign breast lesions was weak and heterogeneous when compared with that seen in most infiltrating carcinomas. In situ carcinomas showed an intermediate pattern of staining. The finding of only small foci of weak positivity for D5 antigen in normal and benign breast disease indicated that there are similarities between the expression of D5 antigen and the presence of oestrogen receptor protein in these tissues. A further similarity was seen with in situ carcinomas, which have been shown to have lower oestrogen receptor content than infiltrating carcinomas and a more heterogeneous staining pattern with D5 than is seen in infiltrating tumours. The importance of these findings remains to be evaluated because the precise nature of D5 antigen and its association with the oestrogen receptor molecule is not fully understood.
利用一种识别D5抗原（一种29,000 kD雌激素受体相关蛋白）的单克隆抗体进行染色模式研究，对象包括7例正常乳腺活检标本、76例良性乳腺活检标本以及12例纯原位乳腺癌。与大多数浸润性癌相比，良性乳腺病变中的染色较弱且不均匀。原位癌呈现出中等程度的染色模式。在正常乳腺和良性乳腺疾病中仅发现小灶性D5抗原弱阳性，这表明这些组织中D5抗原的表达与雌激素受体蛋白的存在之间存在相似性。原位癌还存在另一个相似之处，即已证明其雌激素受体含量低于浸润性癌，且与浸润性肿瘤相比，D5染色模式更不均匀。由于D5抗原的确切性质及其与雌激素受体分子的关联尚未完全明了，这些发现的重要性仍有待评估。
