Cornish J, Vanderwee M, Miller T
Br J Exp Pathol. 1987 Jun;68(3):369-75.
Mucus in the urinary bladder may act as an important intrinsic defence mechanism by shielding the epithelial layer from pathogens. Visualization of any such layer of mucus using electron microscopy has not been possible, as preparative procedures in current use result in the loss or distortion of mucus. We evaluated methods reported to stabilize polysaccharide layers and demonstrated that pretreatment of rat bladder tissue with anti-mucus antiserum, minimized the collapse of the mucus that normally occurred during dehydration of the tissue for electron microscopic examination. Under these conditions, mucus was stabilized as a layer closely adherent to the epithelium.
膀胱中的黏液可能作为一种重要的内在防御机制，通过保护上皮层免受病原体侵害。目前使用的制备程序会导致黏液丢失或变形，因此无法通过电子显微镜观察到任何此类黏液层。我们评估了据报道可稳定多糖层的方法，并证明用抗黏液抗血清预处理大鼠膀胱组织，可最大程度减少组织脱水进行电子显微镜检查时通常发生的黏液塌陷。在这些条件下，黏液稳定地作为紧密附着于上皮的一层存在。
