通过冷冻保存大鼠胃肠道细菌及其微环境关联

Appl Environ Microbiol. 1976 Feb;31(2):304-12. doi: 10.1128/aem.31.2.304-312.1976.

The use of frozen rat gastrointestinal tissue samples for both the recovery of viable bacteria and for observation of microbial communities associated with the tissue was investigated. A decrease of 1 log in lactobacilli, bifidobacteria, and anaerobes was observed when the numbers of bacteria recoverable from frozen tissue (stored 7 to 9 days) were compared to those recoverable from fresh nonfrozen tissue (zero time control). However, freezing did not appear to decrease the numbers of recoverable coliforms. Tissues, cleaved with razor blades after being frozen and stored for 7 to 9 days, showed bacterial communities situated on the mucosa and in the lumen of gastrointestinal specimens. This freezing technique preserved structures not previously observed in the gastrointestinal tract. This indicates that freezing is a good method to use to study such fragile microenvironments.

研究了使用冷冻大鼠胃肠道组织样本恢复活菌以及观察与该组织相关的微生物群落。当将从冷冻组织（储存7至9天）中可恢复的细菌数量与从新鲜非冷冻组织（零时间对照）中可恢复的细菌数量进行比较时，观察到乳酸杆菌、双歧杆菌和厌氧菌数量减少了1个对数。然而，冷冻似乎并未减少可恢复的大肠菌群数量。在冷冻并储存7至9天后用剃须刀片切割的组织，显示出位于胃肠道标本黏膜和管腔中的细菌群落。这种冷冻技术保留了以前在胃肠道中未观察到的结构。这表明冷冻是研究此类脆弱微环境的一种好方法。