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冷藏红细胞的钙通透性。

The calcium permeability of cold-stored erythrocytes.

作者信息

McNamara M K, Wiley J S

出版信息

Br J Haematol. 1987 Jul;66(3):401-3. doi: 10.1111/j.1365-2141.1987.tb06930.x.

DOI:10.1111/j.1365-2141.1987.tb06930.x
PMID:3620359
Abstract

The calcium permeability of erythrocytes was studied by a new technique in which cells were loaded with an intracellular calcium chelator, quin 2, followed by measurement of 45Ca influx. The erythrocyte calcium permeability did not change during cold storage of blood in citrate-phosphate-dextrose for 6 weeks. Previous reports of an increased Ca2+ permeability in cold-stored erythrocytes have been based on an ATP-depletion technique which yields values for Ca2+ influx which are influenced by erythrocyte 2,3DPG levels.

摘要

采用一种新技术研究了红细胞的钙通透性,该技术先使细胞内加载一种细胞内钙螯合剂喹啉-2(quin 2),然后测量45Ca的内流。在柠檬酸盐-磷酸盐-葡萄糖溶液中冷藏6周的血液中,红细胞的钙通透性没有变化。之前关于冷藏红细胞中Ca2+通透性增加的报道是基于一种ATP耗竭技术,该技术得出的Ca2+内流值受红细胞2,3-二磷酸甘油酸(2,3DPG)水平的影响。

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