Genome-wide characterization and sequence polymorphism analyses of cysteine-rich poly comb-like protein in .

Cysteine-rich poly comb-like protein () is a member of cysteine-rich transcription factors that regulates plant growth and development. In the present work, we characterized twelve transcription factors encoding genes in soybean (). Phylogenetic analyses classified genes into six clades. Sequence logos analyses between and amino acid residues exhibited high conservation. The presence of growth and stress-related -acting elements in the upstream regions of highlight their role in plant development and tolerance against abiotic stress. levels showed that experienced limited selection pressure with limited functional divergence arising from segmental or whole genome duplication events. By using the PAN-genome of soybean, a single nucleotide polymorphism was identified in . To perform high throughput genotyping, a kompetitive allele-specific PCR (KASP) marker was developed. Association analyses indicated that allele of (in exon region) was associated with higher thousand seed weight under both water regimes (well-water and water-limited). Taken together, these results provide vital information to further decipher the biological functions of genes in soybean molecular breeding.