Liu Ronglin, Feng Yizhuo, Li Qingyan, Wu Hua, Guo Shengzhou, Li Junnan, Liu Xiaomin, Zhang Yanlin, Tang Xinghao, Cao Shijiang
College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
College of Jun Cao Science and Ecology (College of Carbon Neutrality), Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Plants (Basel). 2025 Mar 5;14(5):803. doi: 10.3390/plants14050803.
The gene family comprises transcription factor genes containing a conserved CRC domain, which is mainly involved in plant development and evolution. Although genes have been widely studied in many plants, little is known about them in woody plants, especially in the endangered species (Hemsl.). In the genome of we identified 11 genes (-) distributed on four chromosomes, with large differences in the number of amino acids. They encode both acidic and alkaline proteins. A phylogenetic analysis showed that these genes can be divided into three subfamilies, A, B, and C, which contain seven, two, and two genes, respectively. Through an interspecific collinearity analysis, we identified homologous genes. A promoter cis-acting element analysis revealed that PbCPPs contain a variety of elements that respond to plant hormones, stress signals, and light and play a role in growth and development, and most genes (except and ) contain MYB binding site elements that regulate drought-induced stress responses, indicating that they play an important role in plant drought resistance. An expression analysis showed that and expression was high in the roots and stems and lower in the leaves, whereas the expression of most of the other genes was low in the roots, stems, and leaves. In addition, six representative genes were detected using qRT-PCR. The results show significant differences in the expression of genes under abiotic stress conditions (drought, cold, and salt), indicating that they play an important role in stress responses. This study preliminarily verified the role of the gene family in different abiotic stress responses, which is of great significance for understanding its mechanism in plant growth and development and stress adaptation.
该基因家族由含有保守CRC结构域的转录因子基因组成，该结构域主要参与植物的发育和进化。尽管该基因已在许多植物中得到广泛研究，但在木本植物中对其了解甚少，尤其是在濒危物种（峨眉拟单性木兰）中。在峨眉拟单性木兰的基因组中，我们鉴定出11个PbCPP基因（PbCPP1 - PbCPP11）分布在四条染色体上，氨基酸数量差异很大。它们编码酸性和碱性蛋白质。系统发育分析表明，这些PbCPP基因可分为三个亚家族，A、B和C，分别包含7个、2个和2个基因。通过种间共线性分析，我们鉴定出了同源的PbCPP基因。启动子顺式作用元件分析表明，PbCPPs含有多种响应植物激素、胁迫信号和光的元件，并在生长发育中发挥作用，并且大多数PbCPP基因（除PbCPP5和PbCPP10外）含有调控干旱诱导胁迫反应的MYB结合位点元件，表明它们在植物抗旱中起重要作用。表达分析表明，PbCPP1和PbCPP2在根和茎中的表达较高，在叶中的表达较低，而其他大多数基因在根、茎和叶中的表达较低。此外，使用qRT-PCR检测了六个代表性的PbCPP基因。结果表明，PbCPP基因在非生物胁迫条件（干旱、寒冷和盐）下的表达存在显著差异，表明它们在胁迫反应中起重要作用。本研究初步验证了PbCPP基因家族在不同非生物胁迫反应中的作用，这对于理解其在植物生长发育和胁迫适应中的机制具有重要意义。
