Yang Youxin, Ahammed Golam Jalal, Wan Chunpeng, Liu Haoju, Chen Rongrong, Zhou Yong
Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables, Collaborative Innovation Center of Postharvest Key Technology and Quality Safety of Fruits and Vegetables, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China.
College of Forestry, Henan University of Science and Technology, Luoyang 471023, China.
Int J Genomics. 2019 Oct 1;2019:6813086. doi: 10.1155/2019/6813086. eCollection 2019.
The gene family is plant-specific and encodes proteins involved in the regulation of multiple biological processes. Here, we identified 15 genes in the watermelon genome, which were divided into four subfamilies (eight JAZs, four ZMLs, two TIFYs, and one PPD) in the phylogenetic tree. The genes were unevenly located on eight chromosomes, and three segmental duplication events and one tandem duplication event were identified, suggesting that gene duplication plays a vital role in the expansion of the gene family in watermelon. Further analysis of the protein architectures, conserved domains, and gene structures provided additional clues for understanding the putative functions of the TIFY family members. Analysis of qRT-PCR and RNA-seq data revealed that the detected genes had preferential expression in specific tissues. qRT-PCR analysis revealed that nine selected genes were responsive to jasmonic acid (JA) and abiotic stresses including salt and drought. JA activated eight genes and suppressed one gene, among which and were the most significantly induced. Salt and drought stress activated nearly all the detected genes to different degrees. These results lay a foundation for further functional characterization of family genes in .
该基因家族是植物特有的，编码参与多种生物过程调控的蛋白质。在此，我们在西瓜基因组中鉴定出15个基因，在系统发育树中它们被分为四个亚家族（8个JAZs、4个ZMLs、2个TIFYs和1个PPD）。这些基因不均匀地分布在8条染色体上，鉴定出了3个片段重复事件和1个串联重复事件，这表明基因重复在西瓜中该基因家族的扩展中起着至关重要的作用。对蛋白质结构、保守结构域和基因结构的进一步分析为理解TIFY家族成员的推定功能提供了额外线索。qRT-PCR和RNA-seq数据分析表明，检测到的基因在特定组织中具有优先表达。qRT-PCR分析表明，9个选定的基因对茉莉酸（JA）以及包括盐和干旱在内的非生物胁迫有响应。JA激活了8个基因并抑制了1个基因，其中[具体基因名称]和[具体基因名称]诱导最为显著。盐和干旱胁迫在不同程度上激活了几乎所有检测到的基因。这些结果为进一步对西瓜中该家族基因进行功能表征奠定了基础。
