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2 型糖尿病大鼠袖状胃切除联合单吻合口十二指肠空肠旁路术后粪便代谢组学分析。

Metabolomics analysis of stool in rats with type 2 diabetes mellitus after single-anastomosis duodenal-ileal bypass with sleeve gastrectomy.

机构信息

Department of Bariatric and Metabolic Surgery, China-Japan Union Hospital of Jilin University, Changchun City, China.

出版信息

Front Endocrinol (Lausanne). 2022 Sep 20;13:1013959. doi: 10.3389/fendo.2022.1013959. eCollection 2022.

DOI:10.3389/fendo.2022.1013959
PMID:36204098
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9530139/
Abstract

BACKGROUND

Single-anastomosis duodenal-ileal bypass with sleeve gastrectomy (SADI-S) is one of the most effective bariatric procedures in the treatment of type 2 diabetes mellitus (T2DM). However, the mechanisms by which SADI-S improves T2DM are not well-known.

OBJECTIVE

To explore the effects of SADI-S on metabolites in the stool of rats with T2DM.

METHODS

Twenty rats were fed on high-fat diet and administered with a low-dose (30mg/kg) of streptozotocin to establish T2DM models. The rats were then randomly assigned to the SADI-S group (n=10) and sham operation group (n=9). Stool samples were collected from all rats at 8 weeks after surgery and stored at -80 °C. Metabolomics analysis was performed to identify differential metabolites through ultra- performance liquid chromatography-mass spectrometry.

RESULTS

At 8-week after surgery, rats of the SADI-S group showed significantly decreased fasting blood glucose, glucose tolerance test 2-hour, glycated haemoglobin, and body weight compared with those of the sham group. A total of 245 differential metabolites were identified between the two groups. Among them, 16 metabolites such as branched-chain amino acids (valine), aromatic amino acid (phenylalanine), bile acid (cholic acid, lithocholic acid, and β-muricholic acid), short-chain fatty acid (isobutyric acid), and phospholipid [lysoPE(17:0), lysoPE(20:3) and lysoPS(16:0)] were associated to the T2DM remission after SADI-S.

CONCLUSION

SADI-S improves T2DM in rats by regulating phenylalanine biosynthesis, valine, phenylalanine, alanine, glutamate, proline, bile acid, and phospholipid metabolism pathways.

摘要

背景

单吻合口十二指肠空肠旁路术联合袖状胃切除术（SADI-S）是治疗 2 型糖尿病（T2DM）最有效的减重手术之一。然而，SADI-S 改善 T2DM 的机制尚不清楚。

目的

探讨 SADI-S 对 T2DM 大鼠粪便代谢物的影响。

方法

将 20 只大鼠给予高脂饮食并给予小剂量（30mg/kg）链脲佐菌素，建立 T2DM 模型。然后将大鼠随机分为 SADI-S 组（n=10）和假手术组（n=9）。所有大鼠于手术后 8 周采集粪便样本，-80°C 保存。通过超高效液相色谱-质谱联用技术进行代谢组学分析，鉴定差异代谢物。

结果

手术后 8 周，SADI-S 组大鼠空腹血糖、葡萄糖耐量试验 2 小时、糖化血红蛋白和体重明显低于假手术组。两组共鉴定出 245 个差异代谢物。其中，16 种代谢物，如支链氨基酸（缬氨酸）、芳香族氨基酸（苯丙氨酸）、胆酸（胆酸、石胆酸和β-鼠胆酸）、短链脂肪酸（异丁酸）和磷脂[溶血磷脂酰乙醇胺（17:0）、溶血磷脂酰甘油（20:3）和溶血磷脂酰丝氨酸（16:0）]与 SADI-S 后 T2DM 缓解有关。

结论

SADI-S 通过调节苯丙氨酸生物合成、缬氨酸、苯丙氨酸、丙氨酸、谷氨酸、脯氨酸、胆酸和磷脂代谢途径改善大鼠 T2DM。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/940b/9530139/731203858a7b/fendo-13-1013959-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/940b/9530139/c2e004c8079d/fendo-13-1013959-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/940b/9530139/69b9f0c03b6d/fendo-13-1013959-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/940b/9530139/101a4c62d0bb/fendo-13-1013959-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/940b/9530139/747e92bb36e5/fendo-13-1013959-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/940b/9530139/8c8c981badae/fendo-13-1013959-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/940b/9530139/731203858a7b/fendo-13-1013959-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/940b/9530139/c2e004c8079d/fendo-13-1013959-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/940b/9530139/69b9f0c03b6d/fendo-13-1013959-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/940b/9530139/101a4c62d0bb/fendo-13-1013959-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/940b/9530139/747e92bb36e5/fendo-13-1013959-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/940b/9530139/8c8c981badae/fendo-13-1013959-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/940b/9530139/731203858a7b/fendo-13-1013959-g006.jpg

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