Zhao Meng, Zhang Hong, Wang Jinming, Shan Dandan, Xu Qian
Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, China.
Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, China.
Food Res Int. 2020 Sep;135:109297. doi: 10.1016/j.foodres.2020.109297. Epub 2020 May 19.
This study aimed to evaluate the intervention effect of whole grain oats (WGO) on a high-fat (HF) diet induced insulin resistance (IR) rat model by metabolomic approach. Sixty rats were randomly allocated into six groups, including the control group, HF group, and four oat-intervention groups. Rats in the control group and HF group were respectively given the standard diet and HF diet respectively. The four oat-intervention groups were fed with a special HF diet, in which sucrose and starch in the HF diet were replaced by whole grain oats powder in different proportions of 25%, 50%, 75%, and 100% respectively. After 8-week feeding, serum samples of all rats were collected for biochemical detection, and the metabolomics analysis was carried out by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). The results showed that the WGO could regulate body weight, blood glucose, blood lipid, and inflammatory response, which indicated that WGO could prevent IR induced by the HF diet, and it was effective enough by 50% WGO replacement. By serum metabolomics analysis, six differential metabolites closely related to WGO intervention on IR were found, and the related metabolic pathways involved energy metabolism, amino acid metabolism, and lipid metabolism.
本研究旨在通过代谢组学方法评估全谷物燕麦（WGO）对高脂（HF）饮食诱导的胰岛素抵抗（IR）大鼠模型的干预效果。将60只大鼠随机分为六组，包括对照组、HF组和四个燕麦干预组。对照组和HF组大鼠分别给予标准饮食和HF饮食。四个燕麦干预组喂食特殊的HF饮食，其中HF饮食中的蔗糖和淀粉分别被全谷物燕麦粉以25%、50%、75%和100%的不同比例替代。喂养8周后，收集所有大鼠的血清样本进行生化检测，并采用超高效液相色谱-四极杆飞行时间质谱联用（UPLC-Q-TOF/MS）进行代谢组学分析。结果表明，WGO可调节体重、血糖、血脂和炎症反应，这表明WGO可预防HF饮食诱导的IR，且50%WGO替代就足够有效。通过血清代谢组学分析，发现了六种与WGO干预IR密切相关的差异代谢物，相关代谢途径涉及能量代谢、氨基酸代谢和脂质代谢。
