Wang Lun, Li Shixing, Jiang Tao
Department of Gastrointestinal Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, China.
Department of Bariatric and Metabolic Surgery, China-Japan Union Hospital of Jilin University, Changchun, China.
Front Microbiol. 2024 May 27;15:1357749. doi: 10.3389/fmicb.2024.1357749. eCollection 2024.
Bariatric and metabolic surgery often leads to significant changes in gut microbiota composition, indicating that changes in gut microbiota after bariatric and metabolic surgery might play a role in ameliorating type 2 diabetes (T2D). However, the effects of single-anastomosis duodenal-ileal bypass with sleeve gastrectomy (SADI-S) on gut microbiota in T2D remain unclear.
To investigate the effects of SADI-S on gut microbiota and glucose metabolism in T2D rats.
Nineteen T2D rats were randomly divided into the SADI-S group ( = 10) and the sham operation with pair-feeding group (sham-PF, = 9). Fecal samples were collected to analyze the gut microbiota composition with 16S ribosomal DNA gene sequencing. The fasting blood glucose and glycated hemoglobin were measured to evaluate the effects of SADI-S on glucose metabolism.
The Chao and ACE index results indicated the richness of the gut microbial community. The ACE and Chao index values were significantly lower in the SADI-S group than in the sham-PF group, indicating that indicating that species richness was significantly lower in the SADI-S group than in the sham-PF group ( < 0.05). Shannon and Simpson indices were used to estimate the species diversity of the gut microbiota. Compared with the sham-PF group, the SADI-S group showed significantly lower Shannon index and higher Simpson index values, indicating that the species diversity was significantly lower in the SADI-S group than in the sham-PF group ( < 0.05). At the genus level, SADI-S significantly changed the abundances of 33 bacteria, including the increased anti-inflammatory bacteria ( and ) and decreased pro-inflammatory bacteria (). SADI-S significantly decreased the fasting blood glucose and glycated hemoglobin levels. The blood glucose level of rats was positively correlated with the relative abundances of 12 bacteria, including , and negatively correlated with the relative abundances of seven bacteria, including .
SADI-S significantly altered the gut microbiota composition of T2D rats, including the increased anti-inflammatory bacteria ( and ) and decreased pro-inflammatory bacteria (Bacteroides). The blood glucose level of rats was positively correlated with the abundances of 12 bacteria, including , but negatively correlated with the relative abundance of 7 bacteria, including . These alternations in gut microbiota may be the mechanism through which SADI-S improved T2D. More studies should be performed in the future to validate these effects.
减肥和代谢手术常常导致肠道微生物群组成发生显著变化，这表明减肥和代谢手术后肠道微生物群的变化可能在改善2型糖尿病（T2D）中发挥作用。然而，单吻合口十二指肠-回肠旁路联合袖状胃切除术（SADI-S）对T2D患者肠道微生物群的影响仍不清楚。
研究SADI-S对T2D大鼠肠道微生物群和糖代谢的影响。
将19只T2D大鼠随机分为SADI-S组（n = 10）和假手术配对喂养组（假手术-PF，n = 9）。收集粪便样本，采用16S核糖体DNA基因测序分析肠道微生物群组成。测量空腹血糖和糖化血红蛋白，以评估SADI-S对糖代谢的影响。
Chao和ACE指数结果表明肠道微生物群落的丰富度。SADI-S组的ACE和Chao指数值显著低于假手术-PF组，表明SADI-S组的物种丰富度显著低于假手术-PF组（P < 0.05）。使用Shannon和Simpson指数估计肠道微生物群的物种多样性。与假手术-PF组相比，SADI-S组的Shannon指数显著降低，Simpson指数值升高，表明SADI-S组的物种多样性显著低于假手术-PF组（P < 0.05）。在属水平上，SADI-S显著改变了33种细菌的丰度，包括抗炎细菌（双歧杆菌属和阿克曼菌属）增加和促炎细菌（拟杆菌属）减少。SADI-S显著降低了空腹血糖和糖化血红蛋白水平。大鼠的血糖水平与12种细菌的相对丰度呈正相关，包括大肠杆菌属，与7种细菌的相对丰度呈负相关，包括阿克曼菌属。
SADI-S显著改变了T2D大鼠的肠道微生物群组成，包括抗炎细菌（双歧杆菌属和阿克曼菌属）增加和促炎细菌（拟杆菌属）减少。大鼠的血糖水平与12种细菌的丰度呈正相关，包括大肠杆菌属，但与7种细菌的相对丰度呈负相关，包括阿克曼菌属。肠道微生物群的这些变化可能是SADI-S改善T2D的机制。未来应进行更多研究以验证这些作用。
