School of Biomedical Engineering, Shenzhen Campus of Sun Yat-sen University, Shenzhen, Guangdong 518107, China.
Biomaterials Research Center, School of Biomedical Engineering, Southern Medical University, Guangzhou, Guangdong 510515, P. R. China.
Biomater Sci. 2022 Nov 8;10(22):6535-6548. doi: 10.1039/d2bm01117d.
() can survive phagocytosis and gain shelter from macrophages in some cases, and the clinical treatment of the intracellular bacterium also encounters the difficulty of traditional antibiotics in entering mammalian cells. In this work, we use mannose-modified bioactive glass nanoparticles decorated with silver nanoparticles (BGNs-Man/Ag) to treat the -induced intracellular infection of macrophages. The results showed that BGNs-Man/Ag could target macrophages, elevate the intracellular ROS levels and drive them toward the M1 phenotype, which was crucial to activate the cell autonomous defence in disposing the intracellular infection. Attractively, BGNs-Man/Ag exhibited higher intracellular bacterial killing efficiency than free vancomycin. For the treatment of subcutaneous abscess, BGNs-Man/Ag significantly increased the population of M1 macrophages at the early stages of the infection site, resulting in enhanced bactericidal activity and improved regeneration of skin tissues. In short, BGNs-Man/Ag can be a promising antibacterial material in treating the -induced intracellular infection of macrophages and subcutaneous abscesses.
（ ）能够在某些情况下逃避吞噬作用并从巨噬细胞中获得庇护，而针对细胞内细菌的临床治疗也遇到了传统抗生素难以进入哺乳动物细胞的困难。在这项工作中，我们使用甘露糖修饰的载银纳米生物玻璃颗粒（BGNs-Man/Ag）来治疗 - 诱导的巨噬细胞内感染。结果表明，BGNs-Man/Ag 可以靶向巨噬细胞，提高细胞内 ROS 水平，并促使它们向 M1 表型转化，这对于激活细胞自主防御以处理细胞内感染至关重要。引人注目的是，BGNs-Man/Ag 表现出比游离万古霉素更高的细胞内杀菌效率。在治疗皮下脓肿方面，BGNs-Man/Ag 显著增加了感染部位早期 M1 巨噬细胞的数量，从而增强了杀菌活性和改善了皮肤组织的再生。总之，BGNs-Man/Ag 有望成为治疗 - 诱导的巨噬细胞内感染和皮下脓肿的抗菌材料。
