Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, 11841, Egypt.
The Regional Center for Mycology and Biotechnology, Al-Azhar University, Nasr City, Cairo, 11787, Egypt.
Biol Trace Elem Res. 2021 Nov;199(11):4225-4236. doi: 10.1007/s12011-020-02561-6. Epub 2021 Jan 2.
Methicillin-resistant Staphylococcus aureus (MRSA) is considered one of the most serious multidrug-resistant bacteria worldwide. MRSA resistance to methicillin antibiotics made vancomycin, the acceptable treatment option. Silver nanoparticles (Ag-NPs) are among the well-known antibacterial substances showing multimode antibacterial action. Therefore, Ag-NPs are appropriate applicants for use in combination with vancomycin in order to augment its antibacterial action. This study aimed to biosynthesize silver nanoparticles and to evaluate its antibacterial activity against MRSA alone and when combined with vancomycin both in vitro and in vivo. Agaricus bisporus is used to reduce the silver nitrate salts in solution to yield silver nanoparticles which was characterized by UV-visible spectrophotometric analysis that shows maximum absorption at 420 nm as a preliminary confirmation for nanoparticles synthesis, Energy-Dispersive Analysis of X-ray (EDX) which confirms the crystalline nature of silver nanoparticles and transmission electron microscopy (TEM) image shows the particles in spherical form with mean size 27.45 nm. The synthesized silver nanoparticles were tested for antibacterial activity against MRSA, and the synergetic effects of the combination of silver nanoparticles and vancomycin were evaluated. The results showed a strong synergistic antibacterial effect between Ag-NPs and vancomycin in vitro with fractional inhibitory concentration 0.37 and in vivo against MRSA strain. The result revealed that mycosynthesized silver nanoparticles (NPs) enhance the in vitro and in vivo antibacterial activity of vancomycin against MRSA. These results suggested that sliver nanoparticles have an effective antibacterial activity against MRSA count, histopathology, and liver enzymes as well as protective immune response specially when combined with vancomycin in the lungs of infected rats with MRSA.
耐甲氧西林金黄色葡萄球菌（MRSA）被认为是世界上最严重的多药耐药细菌之一。MRSA 对甲氧西林抗生素的耐药性使得万古霉素成为可接受的治疗选择。纳米银颗粒（Ag-NPs）是一种众所周知的具有多模式抗菌作用的抗菌物质。因此，Ag-NPs 是与万古霉素联合使用以增强其抗菌作用的合适候选物。本研究旨在生物合成银纳米颗粒，并评估其单独以及与万古霉素联合使用时对 MRSA 的体外和体内的抗菌活性。双孢蘑菇用于将硝酸银盐还原为银纳米颗粒，这通过紫外-可见分光光度分析得到初步确认，该分析显示最大吸收在 420nm 处，作为纳米颗粒合成的初步确认，X 射线能量色散分析（EDX）证实了银纳米颗粒的结晶性质，透射电子显微镜（TEM）图像显示颗粒呈球形，平均尺寸为 27.45nm。合成的银纳米颗粒用于测试对 MRSA 的抗菌活性，并评估银纳米颗粒和万古霉素联合使用的协同效应。结果表明，Ag-NPs 和万古霉素之间在体外具有很强的协同抗菌作用，其半抑制浓度为 0.37，在体内对 MRSA 菌株也具有协同抗菌作用。结果表明，真菌合成的银纳米颗粒（NPs）增强了万古霉素对 MRSA 的体外和体内抗菌活性。这些结果表明，银纳米颗粒对 MRSA 计数、组织病理学和肝酶具有有效的抗菌作用，并且在与万古霉素联合使用时在感染 MRSA 的大鼠肺部中具有保护免疫反应。
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