Institute of Biomaterials, University of Erlangen-Nuremberg, Erlangen, Germany.
School of Clinical Dentistry, The University of Sheffield, Sheffield, United Kingdom.
Mater Sci Eng C Mater Biol Appl. 2019 Oct;103:109764. doi: 10.1016/j.msec.2019.109764. Epub 2019 May 17.
Bioactive glasses (BG) are versatile materials for various biomedical applications, including bone regeneration and wound healing, due to their bone bonding, antibacterial, osteogenic, and angiogenic properties. In this study, we aimed to enhance the antibacterial activity of SiO-CaO mesoporous bioactive glass nanoparticles (MBGN) by incorporating silver (Ag) through a surface modification approach. The modified Ag-containing nanoparticles (Ag-MBGN) maintained spherical shape, mesoporous structure, high dispersity, and apatite-forming ability after the surface functionalization. The antibacterial activity of Ag-MBGN was assessed firstly using a planktonic bacteria model. Moreover, a 3D tissue-engineered infected skin model was used for the first time to evaluate the antibacterial activity of Ag-MBGN at the usage dose of 1 mg/mL. In the planktonic bacteria model, Ag-MBGN exhibited a significant antibacterial effect against both Pseudomonas aeruginosa and Staphylococcus aureus in comparison to non-engineered (Ag-free) MBGN and the blank control. Moreover, Ag-MBGN did not show cytotoxicity towards fibroblasts at the usage dose. However, in the 3D infected skin model, Ag-MBGN only demonstrated antibacterial activity against S. aureus whereas their antibacterial action against P. aeruginosa was inhibited. In conclusion, surface modification by Ag incorporation is a feasible approach to enhance the antibacterial activity of MBGN without significantly impacting their morphology, polydispersity, and apatite-forming ability. The prepared Ag-MBGN are attractive building blocks for the development of 3D antibacterial scaffolds for tissue engineering.
生物活性玻璃（BG）是一种多功能材料，可用于各种生物医学应用，包括骨再生和伤口愈合，因为它具有骨结合、抗菌、成骨和血管生成的特性。在这项研究中，我们旨在通过表面修饰的方法将银（Ag）掺入 SiO-CaO 介孔生物活性玻璃纳米颗粒（MBGN）中，以提高其抗菌活性。经过表面功能化后，改性的含 Ag 纳米颗粒（Ag-MBGN）保持了球形、介孔结构、高分散性和磷灰石形成能力。首先使用浮游细菌模型评估了 Ag-MBGN 的抗菌活性。此外，首次使用 3D 组织工程感染皮肤模型在 1mg/mL 的使用剂量下评估了 Ag-MBGN 的抗菌活性。在浮游细菌模型中，与未经修饰（不含 Ag）的 MBGN 和空白对照组相比，Ag-MBGN 对铜绿假单胞菌和金黄色葡萄球菌均表现出显著的抗菌作用。此外，在使用剂量下，Ag-MBGN 对成纤维细胞没有细胞毒性。然而，在 3D 感染皮肤模型中，Ag-MBGN 仅对金黄色葡萄球菌表现出抗菌活性，而对铜绿假单胞菌的抗菌作用受到抑制。总之，通过 Ag 掺入进行表面修饰是一种可行的方法，可以在不显著影响其形态、多分散性和磷灰石形成能力的情况下提高 MBGN 的抗菌活性。所制备的 Ag-MBGN 是用于组织工程的 3D 抗菌支架的有吸引力的构建块。
