Suárez S S, Osman R A
Biol Reprod. 1987 Jun;36(5):1191-8. doi: 10.1095/biolreprod36.5.1191.
To determine where and when hyperactivation is initiated in vivo, the flagellar curvature ratios (fcr) of mouse sperm within the female reproductive tract were measured from videotape recordings and compared with those of epididymal sperm incubated under capacitating conditions in vitro. The fcrs and linearities of trajectory were significantly lowered after 90 min of incubation in vitro, indicating that hyperactivation had been initiated by that time. The flagellar curvature ratios of sperm at the colliculus tubarius, within the uterotubal junction, and in the isthmus, measured at 1-2 h postcoitus and approximately 1 h before and 1 h after ovulation, were found to have fcrs that were not different from those of sperm incubated for 90 min in vitro. It was concluded that the tract sperm had initiated hyperactivated flagellar bending before the time of ovulation and before entering the oviduct. Only sperm in the lower isthmus 1 h before ovulation had fcrs that were significantly different from sperm incubated for 90 min in vitro, but not from sperm measured at the beginning of incubation in vitro. This could be the result of motility suppression in the lower isthmus.
为了确定体内超激活在何处以及何时开始，从录像记录中测量了雌性生殖道内小鼠精子的鞭毛曲率比（fcr），并将其与在体外获能条件下孵育的附睾精子的鞭毛曲率比进行比较。体外孵育90分钟后，轨迹的fcr和线性显著降低，表明此时已开始超激活。在交配后1 - 2小时以及排卵前约1小时和排卵后1小时测量的输卵管壶腹、输卵管子宫交界处和峡部精子的鞭毛曲率比，发现其fcr与体外孵育90分钟的精子无差异。得出的结论是，生殖道内的精子在排卵前和进入输卵管之前就已开始超激活的鞭毛弯曲。仅排卵前1小时峡部下端的精子fcr与体外孵育90分钟的精子有显著差异，但与体外孵育开始时测量的精子无差异。这可能是峡部下端运动抑制的结果。
