Guangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration, The First Affiliated Hospital of Guangxi Medical, University, Nanning, 530021, China.
Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530021, China.
Biomater Sci. 2022 Nov 8;10(22):6549-6557. doi: 10.1039/d2bm00832g.
Three-dimensional (3D) bioink with favorable printability, strength, and biocompatibility challenged the 3D bioprinting technology in cartilage tissue engineering. Herein, we innovatively fabricated photo-crosslinkable methacrylated konjac glucomannan (KGMMA) as a novel biomaterial ink for 3D extrusion bioprinting in an attempt to construct precisely patterned tissues. Specifically, konjac glucomannan (KGM) was modified by methacrylic anhydride, which is a kind of photoreactive group, to form KGMMA. After UV crosslinking, the printed KGMMA hydrogel formed a covalent crosslinking network with high strength, desired shearing, and swelling and degradation characteristics. The properties of the KGMMA hydrogel could be modulated by changing the contents of MA. The shear-thinning property of the KGMMA biomaterial ink enables excellent printability, which can print different shapes including lattices, hexagons, and flowers. Furthermore, the bioinks support cell growth after being printed with chondrocytes for a culture. Therefore, the biodegradable, injectable, and photo-crosslinkable KGMMA biomaterial ink holds a great promise for cartilage tissue engineering.
三维（3D）生物墨水具有良好的可打印性、强度和生物相容性，这对软骨组织工程中的 3D 生物打印技术提出了挑战。在此，我们创新性地制备了光交联的甲基丙烯酰化魔芋葡甘露聚糖（KGMMA）作为一种新型生物材料墨水，用于 3D 挤出式生物打印，以尝试构建精确图案化的组织。具体而言，魔芋葡甘露聚糖（KGM）被一种光反应基团甲基丙烯酰酐修饰，形成 KGMMA。经 UV 交联后，打印的 KGMMA 水凝胶形成具有高强度、所需剪切和溶胀及降解特性的共价交联网络。通过改变 MA 的含量可以调节 KGMMA 水凝胶的性能。KGMMA 生物材料墨水的剪切稀化特性使其具有优异的可打印性，可打印出不同的形状，包括格子、六边形和花朵。此外，打印后的生物墨水支持软骨细胞的培养。因此，可生物降解、可注射和光交联的 KGMMA 生物材料墨水在软骨组织工程中有很大的应用前景。
