重组蛋白 L：一种通用结合配体的生产、纯化和特性研究。

Recombinant Protein L: Production, Purification and Characterization of a Universal Binding Ligand.

机构信息

Research Division Integrated Bioprocess Development, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Gumpendorfer Strasse 1a, 1060 Vienna, Austria; Alfred Gruber GmbH, Nordstrasse 6, 5301 Eugendorf, Austria.

Research Division Integrated Bioprocess Development, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Gumpendorfer Strasse 1a, 1060 Vienna, Austria.

出版信息

J Biotechnol. 2022 Nov 20;359:108-115. doi: 10.1016/j.jbiotec.2022.10.002. Epub 2022 Oct 4.

DOI:10.1016/j.jbiotec.2022.10.002

PMID:36206851

Abstract

Protein L (PpL) is a universal binding ligand that can be used for the detection and purification of antibodies and antibody fragments. Due to the unique interaction with immunoglobulin light chains, it differs from other affinity ligands, like protein A or G. However, due to its current higher market price, PpL is still scarce in applications. In this study, we investigated the recombinant production and purification of PpL and characterized the product in detail. We present a comprehensive roadmap for the production of the versatile protein PpL in E. coli.

摘要

蛋白 L（PpL）是一种通用的结合配体，可用于检测和纯化抗体和抗体片段。由于与免疫球蛋白轻链的独特相互作用，它与其他亲和配体（如蛋白 A 或 G）不同。然而，由于其目前较高的市场价格，PpL 在应用中仍然稀缺。在这项研究中，我们研究了 PpL 的重组生产和纯化，并对产物进行了详细的表征。我们提出了在大肠杆菌中生产多功能蛋白 PpL 的综合路线图。