Kundacina Ivana, Schobesberger Silvia, Kittler Stefan, Thumfart Helena, Spadiut Oliver, Ertl Peter, Knežević Nikola Ž, Radonic Vasa
University of Novi Sad, BioSense Institute, Dr Zorana Djindjica 1, Novi Sad, 21000, Serbia.
Faculty of Technical Chemistry, TU Wien, Getreidemarkt 9, Vienna, 1060, Austria.
Sci Rep. 2025 Jan 2;15(1):34. doi: 10.1038/s41598-024-83961-9.
Although various sensors specifically developed for target analytes are available, affordable biosensing solutions with broad applicability are limited. In this study, a cost-effective biosensor for detecting human epidermal growth factor receptor 2 (HER2) was developed using custom-made gold leaf electrodes (GLEs). A novel strategy for antibody immobilization on a gold surface, for the first time mediated by protein L and HER2-specific antibody trastuzumab, was examined using commercial screen-printed gold electrodes and GLEs. A self-assembled monolayer of 11-mercaptoundecanoic acid (MUA) was formed on the gold surface, which was used to covalently immobilize protein L. Further binding of trastuzumab to the protein L was employed and HER2 detection was achieved through electrochemical impedance spectroscopy (EIS). The HER2 detection was examined in phosphate-buffered saline (PBS) and supplemented cell culture medium. The modified GLEs showed good specificity and high sensitivity of HER2 detection without any enrichment steps, achieving a limit of detection (LOD) of 1 ng mL in PBS and 2.7 ng mL in cell culture medium, making the proposed immunosensor a cost-effective and sensitive solution for detection in complex biological matrices.
尽管有各种专门为目标分析物开发的传感器，但适用性广泛且价格实惠的生物传感解决方案却很有限。在本研究中，使用定制的金叶电极（GLE）开发了一种用于检测人表皮生长因子受体2（HER2）的经济高效的生物传感器。首次采用由蛋白L和HER2特异性抗体曲妥珠单抗介导的在金表面固定抗体的新策略，使用商业丝网印刷金电极和GLE进行了研究。在金表面形成了11-巯基十一烷酸（MUA）的自组装单分子层，用于共价固定蛋白L。曲妥珠单抗进一步与蛋白L结合，并通过电化学阻抗谱（EIS）实现HER2检测。在磷酸盐缓冲盐水（PBS）和补充的细胞培养基中对HER2检测进行了研究。修饰后的GLE在无需任何富集步骤的情况下对HER2检测显示出良好的特异性和高灵敏度，在PBS中的检测限（LOD）为1 ng/mL，在细胞培养基中的检测限为2.7 ng/mL，这使得所提出的免疫传感器成为一种用于复杂生物基质中检测的经济高效且灵敏的解决方案。
