Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen, 361021, China; University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing, 100049, China.
Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen, 361021, China.
Environ Pollut. 2022 Dec 1;314:120344. doi: 10.1016/j.envpol.2022.120344. Epub 2022 Oct 4.
Pathogens can colonize plant endosphere and, be transferred into human beings through the food chain. However, our understanding of the influences of agricultural activities, such as fertilization, on endophytic microbial communities and human pathogens is still limited. Here, we conducted a microcosm experiment using the combination of 16 S rRNA gene amplicon sequencing and high-throughput qPCR array to reveal the effects of manure fertilization on microbiomes of soils and plants and how such impact is translated into endophytic pathogens. Our results showed that manure fertilization significantly altered soil microbiomes, whereas with less influence on endophytic microbial communities. Soil is a vital source of both bacterial communities and human pathogens for the plant endosphere. The abundance of pathogens was increased both in soils and endosphere under manure fertilization. These findings provide an integrated understanding of the impact of manure fertilization on endophytic pathogens.
病原体可以定植在植物的内共生区，并通过食物链转移到人类体内。然而，我们对于农业活动（如施肥）对内生微生物群落和人类病原体的影响的理解仍然有限。在这里，我们结合 16S rRNA 基因扩增子测序和高通量 qPCR 阵列进行了一个微宇宙实验，以揭示施肥对土壤和植物微生物组的影响，以及这种影响如何转化为内生病原体。我们的结果表明，施肥显著改变了土壤微生物组，而对内生微生物群落的影响较小。土壤是植物内共生区细菌群落和人类病原体的重要来源。在施肥条件下，土壤和内共生区的病原体丰度都增加了。这些发现为理解施肥对内生病原体的影响提供了综合认识。
