State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China.
College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.
Appl Environ Microbiol. 2024 Sep 18;90(9):e0107824. doi: 10.1128/aem.01078-24. Epub 2024 Aug 30.
Plant-associated microbial communities play important roles in agricultural productivity, and their composition has been shown to vary across plant compartments and developmental stages. However, the response of microbial communities within different plant compartments and at different developmental stages to diverse long-term fertilization treatments, as well as their linkages with crop yields, remains underexplored. This study analyzed wheat-associated bacterial communities within various soil and plant compartments under three fertilization treatments throughout the vegetative and reproductive phases. The variance in bacterial community was primarily attributed to compartments, followed by fertilization treatments and developmental stages. The composition of belowground bacterial communities (bulk soil, rhizosphere soil, and root) exhibited stronger responses to fertilization treatments than aboveground compartments (stem and leaf). The composition of belowground bacterial communities responded to fertilization treatments at all developmental stages, and it was significantly correlated with crop yields during the vegetative phase, whereas the aboveground community composition only showed a response to fertilization during the reproductive phase, at which point it was significantly correlated with crop yields. Moreover, during this reproductive phase, the co-occurrence network of aboveground bacterial communities exhibited enhanced complexity, and it contained an increased number of keystone species associated with crop yields, such as spp., spp., and spp. Structural equation modeling indicated that augmenting total phosphorus levels in aboveground compartments could enhance crop yields by increasing the relative abundance of these keystone species during the reproductive phase. These findings highlight the pivotal role of aboveground bacterial communities in wheat production during the reproductive phase.
The developmental stage significantly influences crop-associated bacterial communities, but the relative importance of bacterial communities in different compartments to crop yields across various stages is still not well understood. This study reveals that belowground bacterial communities during the vegetative phase are significantly correlated with crop yields. Notably, during the reproductive phase, the composition of aboveground bacterial communities was significantly correlated with crop yields. During this phase, the complexity and enriched keystone species within the aboveground co-occurrence network underscore their role in boosting crop production. These results provide a foundation for developing microbiome-based products that are phase-specific and promote sustainable agricultural practices.
植物相关微生物群落对农业生产力起着重要作用，其组成已被证明在植物区室和发育阶段之间存在差异。然而，不同植物区室和不同发育阶段的微生物群落对各种长期施肥处理的反应，以及它们与作物产量的联系，仍未得到充分探索。本研究分析了在营养生长期和生殖生长期的三种施肥处理下，不同土壤和植物区室中与小麦相关的细菌群落。细菌群落的变异性主要归因于区室，其次是施肥处理和发育阶段。地下细菌群落（土壤、根际土壤和根系）的组成对施肥处理的响应强于地上区室（茎和叶）。地下细菌群落的组成在所有发育阶段都对施肥处理有响应，并且在营养生长期与作物产量显著相关，而地上群落组成仅在生殖生长期对施肥处理有响应，此时与作物产量显著相关。此外，在生殖期，地上细菌群落的共生网络表现出增强的复杂性，并且包含与作物产量相关的更多关键物种，如 spp.、 spp. 和 spp. 结构方程模型表明，通过在生殖期增加地上区室中的总磷水平，可以通过增加这些关键物种的相对丰度来提高作物产量。这些发现强调了生殖期地上细菌群落对小麦生产的关键作用。
发育阶段显著影响作物相关细菌群落，但不同区室中细菌群落对不同阶段作物产量的相对重要性仍不清楚。本研究表明，营养生长期的地下细菌群落与作物产量显著相关。值得注意的是，在生殖期，地上细菌群落的组成与作物产量显著相关。在这个阶段，地上共生网络的复杂性和丰富的关键物种突出了它们在提高作物产量方面的作用。这些结果为开发基于微生物组的产品提供了基础，这些产品是特定于阶段的，并促进可持续的农业实践。
