Sun Qiaoqian, Zhang Yaoyao, Gao Panpan, Pu Hongchen, Yin Miao, Liang Xiaolong, Yin Wei, Fa Huanbao
School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China.
National-Municipal Joint Engineering Laboratory for Chemical Process Intensification and Reaction, Chongqing University, Chongqing, 400044, China.
Anal Sci. 2023 Jan;39(1):33-42. doi: 10.1007/s44211-022-00193-5. Epub 2022 Oct 8.
In this study, a new enzyme-free glucose sensor was constructed using the transition metal-based composite material. The synthesis of ZIF-67 entailed the addition of NiO with high catalytic performance. Two-dimensional NiO/CoO@C heterojunctions were obtained via pyrolysis of NiO@ZIF-67 in the air at a temperature of 500 ℃. The enzyme-free glucose sensor Nafion/NiO/CoO@C/GCE was constructed by modifying NiO/CoO@C on a glassy carbon electrode (GCE). The performance of the modified electrode was tested via cyclic voltammetry (CV) and a time-current curve (i-t curve). The linear ranges of the modified electrode were 5 -1000 μM and 1.0- 4.0 mM with sensitivities of 690 and 215.4 μA mM cm, respectively. The detection limit was 2.28 μΜ (S/N = 3). The recoveries were in the range of 98.9-99.7% during the detection of real samples. The prepared sensor Nafion/NiO/CoO@C/GCE showed excellent electrocatalytic properties with superb reproducibility, stability and anti-interference capability. The sensor has been successfully utilized to determine glucose in real serum samples.
在本研究中，使用过渡金属基复合材料构建了一种新型无酶葡萄糖传感器。ZIF-67的合成需要添加具有高催化性能的NiO。通过在500℃的空气中对NiO@ZIF-67进行热解，获得了二维NiO/CoO@C异质结。通过在玻碳电极（GCE）上修饰NiO/CoO@C构建了无酶葡萄糖传感器Nafion/NiO/CoO@C/GCE。通过循环伏安法（CV）和计时电流曲线（i-t曲线）测试了修饰电极的性能。修饰电极的线性范围分别为5 - 1000 μM和1.0 - 4.0 mM，灵敏度分别为690和215.4 μA mM cm。检测限为2.28 μΜ（S/N = 3）。在实际样品检测过程中，回收率在98.9 - 99.7%范围内。制备的传感器Nafion/NiO/CoO@C/GCE表现出优异的电催化性能，具有出色的重现性、稳定性和抗干扰能力。该传感器已成功用于测定实际血清样品中的葡萄糖。
