小鼠侧腹部切口致肾缺血再灌注损伤的实验方案。

Protocol for renal ischemia-reperfusion injury by flank incisions in mice.

机构信息

Department of Urology, National Taiwan University Hospital Hsin-Chu Branch, Hsinchu City 300, Taiwan; Department of Urology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei City 100, Taiwan.

Renal Division, Department of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei City 100, Taiwan.

出版信息

STAR Protoc. 2022 Dec 16;3(4):101678. doi: 10.1016/j.xpro.2022.101678. Epub 2022 Oct 7.

DOI:10.1016/j.xpro.2022.101678

PMID:36208451

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9562430/

Abstract

Ischemia-reperfusion injury (IRI) contributes to acute kidney injury (AKI) and development of chronic kidney disease. We describe an IRI protocol for mice via flank incisions approach, using a pedicle clamp to cause ischemic injury. Compared with trans-abdominal approach, it is technically easier with lesser fluid loss and organ injury. Technical challenges during the dissection of renal pedicles are highlighted. For complete details on the execution of this protocol, please refer to Lai et al. (2014).

摘要

缺血再灌注损伤（IRI）可导致急性肾损伤（AKI）和慢性肾脏病的发生。我们通过侧腹部切口方法描述了一种用于小鼠的 IRI 方案，使用蒂夹造成缺血性损伤。与经腹腔方法相比，这种方法技术上更简单，液体丢失和器官损伤更少。本文强调了在分离肾蒂过程中存在的技术挑战。如需了解本方案的详细执行情况，请参考 Lai 等人（2014 年）的研究。