β-胡萝卜素通过调节 KAT7-P15 信号轴、炎症和氧化应激过程发挥抗衰老作用。
Anti-aging effect of β-carotene through regulating the KAT7-P15 signaling axis, inflammation and oxidative stress process.
机构信息
Intervention and Cell Therapy Center, Peking University Shenzhen Hospital, Shenzhen, Guangdong, 518036, People's Republic of China.
College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China.
出版信息
Cell Mol Biol Lett. 2022 Oct 8;27(1):86. doi: 10.1186/s11658-022-00389-7.
BACKGROUND
Research on aging is growing as the elderly make up a greater share of the population, focusing on reversing and inhibiting the aging process. The exhaustion and senescence of stem cells are the fundamental drivers behind aging. β-Carotene has been depicted to have many biological functions, and we speculate that it may have an anti-aging effect.
METHODS
Firstly, the anti-aging property of β-carotene was investigated in vitro using mesenchymal stem cells (MSCs) induced by HO. The anti-aging effect was characterized using Western-bloting, confocal laser scanning microscopy, indirect immunofluorescence, and immunohistochemistry. The anti-aging property was also tested in vivo using aged mice.
RESULTS
The in vitro experiment revealed that β-carotene could relieve the aging of MSCs, as evidenced by a series of aging marker molecules such as p16 and p21. β-Carotene appeared to inhibit aging by regulating the KAT7-P15 signaling axis. The in vivo experiment revealed that β-carotene treatment has significantly down-regulated the aging level of tissues and organs.
CONCLUSIONS
In this work, we explored the anti-aging effect of β-carotene in vivo and in vitro. The experimental results indicate that β-carotene may be an important potential anti-aging molecule, which can be used as a drug or in functional food to treat aging in the future.
背景
随着老年人口在人口中所占比例的增加，针对衰老的研究也在不断增加，重点是逆转和抑制衰老过程。干细胞的衰竭和衰老是衰老的根本驱动因素。β-胡萝卜素有许多生物学功能，我们推测它可能具有抗衰老作用。
方法
首先，使用 HO 诱导的间充质干细胞（MSCs）在体外研究β-胡萝卜素的抗衰老特性。使用 Western-blotting、共聚焦激光扫描显微镜、间接免疫荧光和免疫组织化学来表征其抗衰老作用。还使用老年小鼠进行了体内测试。
结果
体外实验表明，β-胡萝卜素可以缓解 MSCs 的衰老，这一系列衰老标志物分子如 p16 和 p21 为证。β-胡萝卜素似乎通过调节 KAT7-P15 信号轴来抑制衰老。体内实验表明，β-胡萝卜素处理显著下调了组织和器官的衰老水平。
结论
在这项工作中，我们在体内和体外探索了β-胡萝卜素的抗衰老作用。实验结果表明，β-胡萝卜素可能是一种重要的潜在抗衰老分子，将来可作为药物或功能性食品用于治疗衰老。
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