Mechanical and Electrical Engineering College, Hainan University, Haikou, P. R. China.
Shenzhen Academy of Metrology & Quality Inspection, Shenzhen, P. R. China.
Electrophoresis. 2022 Nov;43(21-22):2175-2183. doi: 10.1002/elps.202200102. Epub 2022 Oct 9.
Due to its characteristics of noncontact, non-damage, high flux, and easy-to-achieve flexible manipulation, optically induced dielectrophoresis (ODEP) technology has been employed to manipulate microspherical biological particles, including separation, enrichment, capture, arrangement, and fusion. However, in nature, biomolecules are morphologically diverse, and some of them are rodlike. In order to illustrate the electrodynamics of rodlike particles under the action of ODEP, a transient multi-physical field coupling model of ODEP chip under the hypothesis of electrical double layer thin layer was established in this paper. The arbitrary Lagrangian-Eulerian method is used to track single-rod particle in the strong coupled flow field and electric field simultaneously. The influence of several key factors, including the applied alternating current (AC) electric voltage, the width of optical bright area, and the initial position of particle, on the trajectory of particle center was analyzed in positive dielectrophoresis (DEP) action and negative DEP action, respectively. Especially, the planar motion process of rodlike particles was discussed together. The research results reveal the electrodynamics behavior of rodlike particles based on the action of ODEP, which may provide theoretical support for the further design of rodlike biological cells manipulation chip based on AC ODEP technology in the future.
由于其非接触、无损、高通量和易于实现灵活操作的特点，光诱导介电泳（ODEP）技术已被用于操纵微球形生物颗粒，包括分离、富集、捕获、排列和融合。然而，在自然界中，生物分子形态多样，有些是棒状的。为了说明 ODEP 作用下棒状颗粒的电动力学特性，本文在电双层薄层假设下建立了 ODEP 芯片的瞬态多物理场耦合模型。采用任意拉格朗日-欧拉法同时跟踪单棒状粒子在强耦合流场和电场中的运动。分别在正介电泳（DEP）和负 DEP 作用下，分析了几个关键因素对粒子中心轨迹的影响，包括施加的交流（AC）电压、光学亮区的宽度和粒子的初始位置。特别地，还讨论了棒状粒子的平面运动过程。研究结果揭示了基于 ODEP 作用的棒状粒子的电动力学行为，这可能为未来基于 AC ODEP 技术的棒状生物细胞操作芯片的进一步设计提供理论支持。
