Oesch U, Ammann D, Simon W
Can J Physiol Pharmacol. 1987 May;65(5):885-8. doi: 10.1139/y87-142.
When using microelectrodes for intracellular ion activity studies, some uncertainties such as interference from cytosolic components at the microelectrode, cell damage, and cell contamination may arise. A model, which treats kinetic processes of the loss of carriers from the membrane phase of microelectrodes into the cytosol and cell membrane, is used for an estimation of the extent and time course of contamination by impaled ion-selective microelectrodes. An isolated model cell consisting of a plasma membrane surrounding a cytosolic milieu is assumed. The results of its considerations represent a worst case situation, in which significant contamination of the cell membrane of such a small isolated single cell might occur during time periods of electrophysiological experiments. In more complex situations, such as in intact tissues, the equilibrium membrane concentrations may be substantially less.
在使用微电极进行细胞内离子活性研究时，可能会出现一些不确定因素，如微电极处细胞溶质成分的干扰、细胞损伤和细胞污染。一个模型用于估计刺入的离子选择性微电极的污染程度和时间进程，该模型处理从微电极膜相到细胞质和细胞膜的载体损失的动力学过程。假设一个由围绕细胞质环境的质膜组成的分离模型细胞。其考虑结果代表了一种最坏情况，即在电生理实验期间，如此小的分离单细胞的细胞膜可能会发生显著污染。在更复杂的情况下，如在完整组织中，平衡膜浓度可能会低得多。
